Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt

Hassuna, Noha A.; Koriem, Nehal E.; Zaki, Shaimaa Hassouna; Abd El Fattah, Medhat; Abdelmoaty, Amani Mohammed; Ibrahem, Reham A.

doi:10.21608/mid.2024.279851.1883

	Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt
Microbes and Infectious Diseases
Article 23, Volume 5, Issue 3, August 2024, Pages 1127-1138 PDF (845.4 K)
Document Type: Original Article
DOI: 10.21608/mid.2024.279851.1883
Authors
Noha A. Hassuna¹; Nehal E. Koriem²; Shaimaa Hassouna Zaki^* ¹; Medhat Abd El Fattah²; Amani Mohammed Abdelmoaty³; Reham A. Ibrahem⁴
¹Medical Microbiology and Immunology Department, Faculty of Medicine, Minia University, Minia, Egypt
²Botany and Microbiology, Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt
³Pharmaceutics, Faculty of Pharmacy, Beni-Suef University, Beni-Suef University, Beni Suef, Egypt
⁴Microbiology and Immunology Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Abstract
Background: The last-resort classes of antibiotics with the best success in treating severe Pseudomonas aeruginosa (P. aeruginosa) infections caused by drug-resistant P. aeruginosa are carbapenems. So, we aimed to assess the frequency of carbapenem-resistant genes in P. aeruginosa. Methods: Samples isolated from critically ill patients at Intensive Care Units (ICUs) and surgical wards in Minia, Egypt were examined for imipenem-resistance phenotypically by Modified Hodge test (MHT) and Modified carbapenem inactivation method (mCIM) test and genotypically by PCR. Results: Fifty isolates (64%) of P. aeruginosa were resistant to carbapenems (imipenem and meropenem), of which: 16 (32%) were (MHT) positive and 26 (52 %) were (mCIM)-positive. Regarding resistance genes, bla_IMP, bla_VIM, and bla_KPC were detected in 14%, 20%, and 48% of the carbapenem resistant Pseudomonas aeruginosa (CRPA) isolates; respectively, while bla_GIM, bla_SIM, bla_NDM-1and bla_SPM-1 were not detected. Conclusion: There is a high prevalence of multi-drug resistant (MDR) strains of P. aeruginosa. Doctors should pay attention to carbapenem resistance. mCIM test was much more sensitive than MHT for phenotypic detection of CRPA isolates. bla_KPCgene was the most frequently detected gene.
Keywords
Keywords: P. aeruginosa; mCIM; MHT; carbapenemases; PCR

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