Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt
Hassuna, N., Koriem, N., Zaki, S., Abd El Fattah, M., Abdelmoaty, A., Ibrahem, R. (2024). Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt. EKB Journal Management System, 5(3), 1127-1138. doi: 10.21608/mid.2024.279851.1883
Noha A. Hassuna; Nehal E. Koriem; Shaimaa Hassouna Zaki; Medhat Abd El Fattah; Amani Mohammed Abdelmoaty; Reham A. Ibrahem. "Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt". EKB Journal Management System, 5, 3, 2024, 1127-1138. doi: 10.21608/mid.2024.279851.1883
Hassuna, N., Koriem, N., Zaki, S., Abd El Fattah, M., Abdelmoaty, A., Ibrahem, R. (2024). 'Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt', EKB Journal Management System, 5(3), pp. 1127-1138. doi: 10.21608/mid.2024.279851.1883
Hassuna, N., Koriem, N., Zaki, S., Abd El Fattah, M., Abdelmoaty, A., Ibrahem, R. Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt. EKB Journal Management System, 2024; 5(3): 1127-1138. doi: 10.21608/mid.2024.279851.1883

Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt

Microbes and Infectious Diseases
Article 23, Volume 5, Issue 3, August 2024, Page 1127-1138  XML PDF (845.4 K)
Document Type: Original Article
DOI: 10.21608/mid.2024.279851.1883
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Authors
Noha A. Hassunaorcid 1; Nehal E. Koriem2; Shaimaa Hassouna Zaki email orcid 1; Medhat Abd El Fattah2; Amani Mohammed Abdelmoaty3; Reham A. Ibrahem4
1Medical Microbiology and Immunology Department, Faculty of Medicine, Minia University, Minia, Egypt
2Botany and Microbiology, Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt
3Pharmaceutics, Faculty of Pharmacy, Beni-Suef University, Beni-Suef University, Beni Suef, Egypt
4Microbiology and Immunology Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Abstract
Background:  The last-resort classes of antibiotics with the best success in treating severe Pseudomonas aeruginosa (P. aeruginosa) infections caused by drug-resistant P. aeruginosa are carbapenems. So, we aimed to assess the frequency of carbapenem-resistant genes in P. aeruginosa. Methods: Samples isolated from critically ill patients at Intensive Care Units (ICUs) and surgical wards in Minia, Egypt were examined for imipenem-resistance phenotypically by Modified Hodge test (MHT) and Modified carbapenem inactivation method (mCIM) test and genotypically by PCR. Results: Fifty isolates (64%) of P. aeruginosa were resistant to carbapenems (imipenem and meropenem), of which: 16 (32%) were (MHT) positive and 26 (52 %) were (mCIM)-positive. Regarding resistance genes, blaIMP, blaVIM, and blaKPC were detected in 14%, 20%, and 48% of the carbapenem resistant Pseudomonas aeruginosa (CRPA) isolates; respectively, while blaGIM, blaSIM, blaNDM-1 and blaSPM-1 were not detected.  Conclusion: There is a high prevalence of multi-drug resistant (MDR) strains of P. aeruginosa. Doctors should pay attention to carbapenem resistance. mCIM test was much more sensitive than MHT for phenotypic detection of CRPA isolates. blaKPC gene was the most frequently detected gene.
Keywords
Keywords: P. aeruginosa; mCIM; MHT; carbapenemases; PCR
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