Interferon-induced transmembrane protein 3 (IFITM3) gene rs12252 polymorphism and COVID-19 severity in a cohort of Egyptian patients | ||
| ALEXMED ePosters | ||
| Article 1, Volume 6, Issue 2, April 2024, Pages 21-22 | ||
| Document Type: Preliminary preprint short reports of original research | ||
| DOI: 10.21608/alexpo.2024.287800.1832 | ||
| Authors | ||
| Nermine ‎ Hossam ELDin Zakaria1; Ehab Mahmoud El Reweny2; Raghda Saad Zaghloul3; Israa Hassan Gebril* 4 | ||
| 1Department of Clinical and chemical pathology‎, Faculty of Medicine, Alexandria University | ||
| 2Department of Critical Care Medicine, Faculty of Medicine, Alexandria University | ||
| 3Department of Clinical & chemical Patholohy**, Faculty of Medicine, Alexandria University, Egypt | ||
| 4Department of clinical and chemical pathology | ||
| Abstract | ||
| The pandemic of Coronavirus disease 2019 (COVID-19)resulted in about 6 million deaths around the world till December 2022 according to the reports of the World Health Organization (WHO). Extensive studies have explored the innate defense mechanisms and cellular proteins involved in immunity against the infection of coronaviruses. Interferon-induced transmembrane proteins (IFITMs) have been identified as key interferon-stimulating genes (ISGs) that interfere with viral endosomal membrane fusion and the infectivity of nascent virions. The rs12252 single nucleotide polymorphism (SNP) within the first exon of the IFITM3 gene has been associated with increased severity in some viruses such asinfluenza virus and Human immunodeficiency virus (HIV). Aim of the work: The study aimed to investigate the association between interferon-induced transmembrane protein 3 gene (IFITM3) rs12252 polymorphism with disease severity in coronavirus disease. Patients and Methods: This case-control study was conducted on 87 laboratory-confirmed COVID-19 patients from Alexandria University Hospitalsand 75 healthy controls. The patient group was further subdivided into 3 groups; mild, moderate, and severe including 23, 36 and 28 patients respectively. Laboratory confirmation of the cases was defined as a positive COVID-19 result of real-time reverse-transcription polymerase chain reaction assay of nasopharyngeal swab specimens. Blood samples were collected on K2EDTA tubes for genotyping of IFITM3 gene SNP (rs12252).Detection of IFITM3 rs12252 SNP was performed via real-time PCR using TaqMan SNP genotyping assay. | ||
| Keywords | ||
| Coronavirus disease (COVID-19) severity; interferon- induced transmembrane protein 3 gene; SNP | ||
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