Design of Potentiometric Sensor Based on Molecularly-imprinted Polymer for Direct Detection of Levofloxacin in Plasma for Point-of-Care Applications
Mahmoud, A., Saleh, H., Fawzy, M., Reda, A., Bahgat, E. (2024). Design of Potentiometric Sensor Based on Molecularly-imprinted Polymer for Direct Detection of Levofloxacin in Plasma for Point-of-Care Applications. EKB Journal Management System, 33(1), 38-48. doi: 10.21608/zjps.2024.280479.1069
Amr M. Mahmoud; Hanaa Saleh; Michael Gamal Fawzy; Alaa Reda; Eman A. Bahgat. "Design of Potentiometric Sensor Based on Molecularly-imprinted Polymer for Direct Detection of Levofloxacin in Plasma for Point-of-Care Applications". EKB Journal Management System, 33, 1, 2024, 38-48. doi: 10.21608/zjps.2024.280479.1069
Mahmoud, A., Saleh, H., Fawzy, M., Reda, A., Bahgat, E. (2024). 'Design of Potentiometric Sensor Based on Molecularly-imprinted Polymer for Direct Detection of Levofloxacin in Plasma for Point-of-Care Applications', EKB Journal Management System, 33(1), pp. 38-48. doi: 10.21608/zjps.2024.280479.1069
Mahmoud, A., Saleh, H., Fawzy, M., Reda, A., Bahgat, E. Design of Potentiometric Sensor Based on Molecularly-imprinted Polymer for Direct Detection of Levofloxacin in Plasma for Point-of-Care Applications. EKB Journal Management System, 2024; 33(1): 38-48. doi: 10.21608/zjps.2024.280479.1069

Design of Potentiometric Sensor Based on Molecularly-imprinted Polymer for Direct Detection of Levofloxacin in Plasma for Point-of-Care Applications

Zagazig Journal of Pharmaceutical Sciences
Volume 33, Issue 1, June 2024, Page 38-48  XML PDF (690.97 K)
Document Type: Original Article
DOI: 10.21608/zjps.2024.280479.1069
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Authors
Amr M. Mahmoudorcid 1; Hanaa Salehorcid 2; Michael Gamal Fawzyorcid 3; Alaa Reda email orcid 3; Eman A. Bahgatorcid 2
1Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini Street, 11562 Cairo, Egypt
2Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
3Pharmaceutical Chemistry Department, Faculty of Pharmacy, Egyptian Russian University, Badr City, Cairo 11829, Egypt
Abstract
Abstract
Background: With the goal of "personalizing" the patient's dosage regimen, the concentration of the drug is measured in biological fluids as a part of therapeutic drug monitoring (TDM). A reproducible and disposable molecularly imprinted polymer (MIP)-based potentiometric sensor was constructed as a TDM analysis platform for levofloxacin (LVF) assay in spiked plasma samples and pharmaceutical formulation.
Materials and methods: In order to obtain high selectivity for the drug, a host guest interaction technique was employed, using methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross linker in the presence of the template, LVF. Fourier transform infrared (FT-IR) spectroscopy was used for polymer structural characterization.
Results: the sensor responded quickly within 3-5 seconds in the pH range (3.0 – 6.0). Within the concentration range of (1 × 10-5 - 1 × 10-2 M), the potential profile showed a linear relationship with limit of detection (LOD) of 7.41 × 10-6 M. Significantly, the selectivity towards LVF was promoted using the MIP modified sensor. The sensor was successfully employed for LVF assay in spiked plasma samples and pharmaceutical dosage form without any interference from any common additives or excipients. Using analytical eco-scale and Green Analytical Procedure Index (GAPI) techniques, the suggested method's greenness was assessed, and it showed outstanding green analysis.
Conclusion: the obtained results showed that the developed MIP-based sensor is selective, simple, easily handled, and rapid for LVF assay in pure form, spiked plasma samples, and tablets with good selectivity, accuracy, and precision.
Keywords
Molecularly-imprinted polymer; Ion-selective electrodes; Therapeutic drug monitoring; Levofloxacin; plasma samples
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