Genetic evaluation of CD28 rs3116496 in Egyptian children with Type 1 Diabetes (T1D) | ||
| Research Journal of Applied Biotechnology | ||
| Article 3, Volume 8, Issue 1, June 2022, Pages 27-34 PDF (272.38 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/rjab.2022.357079 | ||
| Authors | ||
| Yasser B.M. Ali* 1; Dina R. Elkera2; Basima A. Elakhras1; Heba S. El-Mahdy2; Mohamed Osman3 | ||
| 1Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt. | ||
| 2Pediatric Department, Faculty of Medicine, Tanta University, Egypt. | ||
| 3Department of Bioinformatics, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt. | ||
| Abstract | ||
| The genetic contribution of Cluster of Differentiation 28 (CD28) toward type 1 diabetes (T1D) was investigated in several ethnic groups.The genotyping of CD28 rs3116496 (+17 T/C) polymorphism was performed in the Egyptian population (101 T1D patients and 100 healthy controls) by using a single-stranded-polymorphism-PCR (SSP-PCR). Despite the comparable genetic distribution of CD28 between the two groups, patients had significantly higher CT/TT against CC than healthy individuals (P<0.05 and OR=3.63). Sodium levels were decreased in patients with the CC genotype (CD28 C/T), whereas urea levels were raised in TT genotype patients. Our present study found that there may be a link between Egyptian children's T1D risk and CD28 variants. | ||
| Keywords | ||
| Keywords: Diabetic; Single nucleotide polymorphisms; PCR; CD28 | ||
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