Two eco-friendly spectrophotometric methods for the concurrent estimation of diacerein and meloxicam: greenness and whiteness assessment
El-Kafrawy, D., Abo-Gharam, A., Abdel-Khalek, M., Belal, T. (2024). Two eco-friendly spectrophotometric methods for the concurrent estimation of diacerein and meloxicam: greenness and whiteness assessment. EKB Journal Management System, 1(2), 86-99. doi: 10.21608/japs.2024.286224.1022
Dina Salah El-Kafrawy; Amira Abo-Gharam; Magdy Abdel-Khalek; Tarek Belal. "Two eco-friendly spectrophotometric methods for the concurrent estimation of diacerein and meloxicam: greenness and whiteness assessment". EKB Journal Management System, 1, 2, 2024, 86-99. doi: 10.21608/japs.2024.286224.1022
El-Kafrawy, D., Abo-Gharam, A., Abdel-Khalek, M., Belal, T. (2024). 'Two eco-friendly spectrophotometric methods for the concurrent estimation of diacerein and meloxicam: greenness and whiteness assessment', EKB Journal Management System, 1(2), pp. 86-99. doi: 10.21608/japs.2024.286224.1022
El-Kafrawy, D., Abo-Gharam, A., Abdel-Khalek, M., Belal, T. Two eco-friendly spectrophotometric methods for the concurrent estimation of diacerein and meloxicam: greenness and whiteness assessment. EKB Journal Management System, 2024; 1(2): 86-99. doi: 10.21608/japs.2024.286224.1022

Two eco-friendly spectrophotometric methods for the concurrent estimation of diacerein and meloxicam: greenness and whiteness assessment

Journal of Advanced Pharmaceutical Sciences
Volume 1, Issue 2, June 2024, Pages 86-99    PDF (810.01 K)
Document Type: Original Article
DOI: 10.21608/japs.2024.286224.1022
Authors
Dina Salah El-Kafrawy* 1; Amira Abo-Gharam2; Magdy Abdel-Khalek2; Tarek Belal3
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Alexandria, Alexandria, Egypt.
2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
3Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
Abstract
The current study represents the concurrent analysis of diacerein and meloxicam in their binary mixture by two simple spectrophotometric methods, for the first time without prior separation, namely the dual-wavelength and the zero-crossing derivative spectrophotometry methods. Firstly, the dual wavelength method involves the selection of two wavelengths at the original absorption spectra for each drug in such a way that the subtraction of absorbance readings equals zero for the second drug. Wavelengths at 256.0 and 308.5 nm were chosen for the estimation of diacerein where meloxicam showed equal absorption values. Similarly, wavelengths at 357.0 and 281.0 nm were selected for the determination of meloxicam without any contribution of diacerein. Secondly, the other method depends on derivative spectrophotometry with zero-crossing measurement to solve the spectral overlap problem where diacerein was easily assayed using 2D amplitudes at 265.5 nm with no contribution from meloxicam.
Also, meloxicam was determined by calculating the sum of the absolute values of 1D amplitudes at 339 and 395 nm, where diacerein was zero-crossing at these wavelengths. The ICH guidelines were used to validate the analytical performance of the adopted methods. For the two adopted methods, diacerein and meloxicam showed perfect linearities in the ranges 2-14 µg/mL and 3-24 µg/mL, respectively, with high correlation coefficients (≥ 0.99975). The validated procedures were effectively used in the concurrent quantification of both drugs either in bulk powders, synthetic mixtures, or laboratory-prepared capsules. Finally, the greenness and whiteness of the adopted methods were assessed and compared among other reported procedures. 

Highlights
  • Two spectrophotometric methods named the dual wavelength and zero-crossing derivative spectrophotometry techniques were investigated for the concurrent estimation of DCN and MLX in their combined formulation.
  • The developed methods were validated in accordance with ICH guidelines and can be easily applied for the determination of this binary mixture in quality control units.
  • The suggested methods possess greenness and whiteness properties according to GAPI, AGREE, and RGB approaches.
Keywords
Spectrophotometric determination; Diacerein; Meloxicam; Greenness and whiteness assessment; Combined capsules
Supplementary Files
References
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