Potential Effect of Thymoquinone on HNO-97 Cell Line
Eloraby, D., Elgayar, S., Mokbel, M., El-Bolok, A. (2024). Potential Effect of Thymoquinone on HNO-97 Cell Line. EKB Journal Management System, 34(2), 23-30. doi: 10.21608/asdj.2024.274346.1222
Dina Eloraby; Sherif Elgayar; Marwa Mokbel; Amr El-Bolok. "Potential Effect of Thymoquinone on HNO-97 Cell Line". EKB Journal Management System, 34, 2, 2024, 23-30. doi: 10.21608/asdj.2024.274346.1222
Eloraby, D., Elgayar, S., Mokbel, M., El-Bolok, A. (2024). 'Potential Effect of Thymoquinone on HNO-97 Cell Line', EKB Journal Management System, 34(2), pp. 23-30. doi: 10.21608/asdj.2024.274346.1222
Eloraby, D., Elgayar, S., Mokbel, M., El-Bolok, A. Potential Effect of Thymoquinone on HNO-97 Cell Line. EKB Journal Management System, 2024; 34(2): 23-30. doi: 10.21608/asdj.2024.274346.1222

Potential Effect of Thymoquinone on HNO-97 Cell Line

Ain Shams Dental Journal
Article 4, Volume 34, Issue 2, June 2024, Page 23-30  XML PDF (1.35 MB)
Document Type: Original articles
DOI: 10.21608/asdj.2024.274346.1222
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Authors
Dina Eloraby email orcid 1; Sherif Elgayarorcid 2; Marwa Mokbelorcid 3; Amr El-Bolokorcid 2
1Oral and Maxillofacial Pathology Department, Faculty of oral and dental medicine, Misr International University, Obour 19648, Egypt
2Faculty of Dentistry, Minia University
3Faculty of Oral and Dental Medicine, Misr International University
Abstract
Abstract:
Aim: The aim of the current study was to assess the effect of thymoquinone (TQ) on tongue squamous cell carcinoma cell line (HNO-97).
Materials and Methods: Tongue squamous carcinoma cell line (HNO-97) was obtained, and maintained in cultured flasks and were divided into; Group Ι (Control): Untreated (HNO-97) cells and three treated groups: HNO-97-treated cells with three different concentrations of TQ. All cells were incubated for 24 h. The viability of HNO-97 cells treated with TQ was determined 24 h post treatment. Determination of the effect of TQ on HNO-97 cell line was done by Methyl Thiazol Tetrazolium (MTT) Cytotoxicity Assay, flow cytometry cell cycle assay, and ELISA test by detection of TNF-α.
Results: MTT assay showed that TQ cytotoxicity was in dose dependent. The mean percentage of viability of the treated cells decreased in a dose dependent manner. Moreover, TQ treatment induced reduction in the percentage of cells in the G1 to S phases of the cell cycle and increased the percentage of cancer cells in the G2/M phase. Furthermore, TQ treatment significantly decreased TNF-α level in a dose dependent manner.
Conclusion: The effect of TQ decreased the viability of Tongue Squamous Cell Carcinoma Cell Line in a dose dependent manner.
Keywords
Carcinoma; Squamous; Thymoquinone; Tongue
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