Potential Effect of Thymoquinone on HNO-97 Cell Line | ||
| Ain Shams Dental Journal | ||
| Article 4, Volume 34, Issue 2, June 2024, Pages 23-30 PDF (1.35 M) | ||
| Document Type: Original articles | ||
| DOI: 10.21608/asdj.2024.274346.1222 | ||
| Authors | ||
| Dina Eloraby* 1; Sherif Elgayar2; Marwa Mokbel3; Amr El-Bolok2 | ||
| 1Oral and Maxillofacial Pathology Department, Faculty of oral and dental medicine, Misr International University, Obour 19648, Egypt | ||
| 2Faculty of Dentistry, Minia University | ||
| 3Faculty of Oral and Dental Medicine, Misr International University | ||
| Abstract | ||
| Abstract: Aim: The aim of the current study was to assess the effect of thymoquinone (TQ) on tongue squamous cell carcinoma cell line (HNO-97). Materials and Methods: Tongue squamous carcinoma cell line (HNO-97) was obtained, and maintained in cultured flasks and were divided into; Group Ι (Control): Untreated (HNO-97) cells and three treated groups: HNO-97-treated cells with three different concentrations of TQ. All cells were incubated for 24 h. The viability of HNO-97 cells treated with TQ was determined 24 h post treatment. Determination of the effect of TQ on HNO-97 cell line was done by Methyl Thiazol Tetrazolium (MTT) Cytotoxicity Assay, flow cytometry cell cycle assay, and ELISA test by detection of TNF-α. Results: MTT assay showed that TQ cytotoxicity was in dose dependent. The mean percentage of viability of the treated cells decreased in a dose dependent manner. Moreover, TQ treatment induced reduction in the percentage of cells in the G1 to S phases of the cell cycle and increased the percentage of cancer cells in the G2/M phase. Furthermore, TQ treatment significantly decreased TNF-α level in a dose dependent manner. Conclusion: The effect of TQ decreased the viability of Tongue Squamous Cell Carcinoma Cell Line in a dose dependent manner. | ||
| Keywords | ||
| Carcinoma; Squamous; Thymoquinone; Tongue | ||
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