Down-regulation of MicroRNA-137 Improves Proliferation of Oral Squamous Cell Carcinoma by Up-regulation of NF-Y | ||
| Ain Shams Dental Journal | ||
| Volume 34, Issue 2, June 2024, Pages 241-250 PDF (1.57 M) | ||
| Document Type: Original articles | ||
| DOI: 10.21608/asdj.2024.288126.1275 | ||
| Authors | ||
| Amira Abdel Azeem Saber* 1; Safaa Marzouk Tohamy2; Maii Ibrahim Ali Sholqamy3; Loay Abdel-latef mohamed4 | ||
| 1Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University Assiut ,Egypt | ||
| 2Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University | ||
| 3Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Minia, Egypt | ||
| 4Oral and Maxillofacial Pathology, Faculty of Dentistry, minia University,minia,Egypt | ||
| Abstract | ||
| ABSTRACT: Aim: The purpose of our study was to investigate the impact of miR-137 suppression on the growth of the OECM-1 human tongue SCC cell line. In addition, by targeting the NF-Y gene, we also studied the molecular mechanism of miR-137. Methods: The (OECM-1) & negative control (SCC-15) cells were transfected with microRNA137 inhibitor, MTT assay was utilized to ascertain the cytotoxic impact. SYBER green-based quantitative polymerase chain reaction was used to determine the expression level of both the miR-137 and NF-Y genes in treated and untreated cells. The collected data were subjected to One-way analysis of variance (ANOVA) and Tukey's multiple comparisons test. RESULTS: According to our research, OECM-1 cells transfected with an inhibitor of miR-137 significantly increased their ability to proliferate when compared to an untreated samples & negative control. Also, we reported that miR-137 is down regulated in OSCC and suppression of miR-137 significantly associated with 1.4 folds increase in NF-Y gene, compared to untreated OECM-1 cells. CONCLUSION: MiR-137 is down regulated in OSCC cell lines compared to normal control & its inhibition has a significant increase in cell proliferation which reflects the tumor suppressor role of miR-137 in OSCC. In addition, its inhibition is associated with marked increase in the expression of NF-Y which suggested NF-Y a new target for miR-137. | ||
| Keywords | ||
| KEYWORDS: OSCC; MiR-137; NF-Y; Cell line | ||
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