Five isolated phytochemicals from Iraqi Medicago sativa with tissue cultured comparative study by reverse-phase High Performance Liquid Chromatography (RP-HPLC) | ||||
| Egyptian Journal of Health Care | ||||
| Volume 15, Issue 2, June 2024, Page 1029-1037 PDF (622.37 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejhc.2024.359308 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Firas S. Al-Dabbagh | ||||
| Anesthesia Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq. | ||||
| Abstract | ||||
| Medicago sativa L. (alfalfa) is one of the most cultivated plants which has high nutritional value as fodder since antiquity and to treat many health disorders related to secondary metabolites with antioxidant and anti-inflammatory effects. For the first time, a comparative study was applied for bioactive constituents of tissue cultured and their ordinary counterparts of Iraqi Medicago sativa. Two types of agriculture techniques were employed, plant tissue cultured (three different tissue culture types) and ordinary agriculture (two different aerial parts and growth stages). The reverse - phase high-performance liquid chromatography applied for qualitative and quantitative of different phytochemicals in this plant giving an idea about the differences in the availabilities of these phytochemicals like steroids (Beta-sitosterol, Stigmasterol), phenolic acids(Caffeic acid, Chlorogenic acid, Gallic acid), and flavonoids(Myricetine, Naringinine) by the differentiated techniques. | ||||
| Keywords | ||||
| Medicago sativa L; tissue culture; revese – phase high-performance liquid chromatography (RP-HPLC) | ||||
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