Genotypic characterization of Brucella Spp.isolated from sheep and goats | ||||
| Benha Veterinary Medical Journal | ||||
| Article 3, Volume 35, Issue 1, September 2018, Page 22-29 PDF (165.81 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2018.35938 | ||||
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| Authors | ||||
| Ashraf Abdel-Tawab* 1; Mohsen Agag2; Mahmoud Hamdy3; Gamal Wareth4; Fatma El-Hofy1; Mohamed Ramadan5 | ||||
| 1Dept. of bacteriology, Immunology and Mycology Benha Univ. | ||||
| 2Dept. of TheriogenologyBenha Univ. | ||||
| 3Animal Health Institute,Dokki, Giza. | ||||
| 4Dept. of Pathology Benha Univ. | ||||
| 5Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Jena, Germany | ||||
| Abstract | ||||
| Brucellosis is a major infectious zoonotic disease affecting animal and human.In animal, the disease is causing great economic losses such as abortion, still birth as well as infertility in both male and female.A cross sectional study carried out from November 2016 to May 2017 to estimate the seroprevalence of brucellosis in sheep and goatsin Benha.A total of 38 animals (20 sheep, 18 goats)were investigated. 19 serum samples (8 from sheep and 11 from goats) subjected for serological examination. The results showed that,5/8(62.5%) and 9/11(81.8%) from sheep and goats, respectively, were positive by Rose Bengal Plat test(RBPT), Buffered Acidified Plat Antigen test(BABA)and Rivanol test (RIV test).Atotal of 34 lymph nodes from seropositive sheep and goats were examined bacteriologically. 3/20 (15%) and 4/14 (28.57%) were culture positive for Brucellarespectively. None of vaginal swabs revealed culture positive results.All isolates were confirmed genetically as Brucella spp. by PCR and all strains identified asB. melitensis by AMOS- PCR. | ||||
| Keywords | ||||
| Brucella spp; PCR; AMOS-PCR; Sheep and goats | ||||
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