Effect of Melatonin on some Inflammatory Markers in L-arginine Induced acute Pancreatitis in Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 11, Volume 31, Issue 1, December 2011, Page 147-164 PDF (535.73 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2011.35978 | ||||
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| Authors | ||||
| Hala Hamouda* 1; Ahmed Abd-Allah1; Ghada Abd El Alem1; Omnia El-Deeb1; Kareema El-Desoky2 | ||||
| 1Department of Medical Biochemistry, Faculty of Medicine, Tanta University | ||||
| 2Department of Pathology, Faculty of Medicine, Tanta University | ||||
| Abstract | ||||
| Objective: Acute pancreatitis is an inflammatory disease of the pancreas resulting in significant morbidity and mortality, due to distant organ failure. Melatonin exerts complex physiological and pharmacological effects on multiple systems and organs. Aim of the study: The present study was designed to investigate the effects of melatonin pre- and post-treatment with L-arginine (L-Arg.) on some inflammatory markers in acute pancreatitis in rats. Materials and methods: The present study was carried out on 75 white albino rats which were randomly classified into five equal groups; group I (control group); group II (L-arginine injected group); group III (melatonin pre-treatment with L-arginine); group IV (melatonin post-treatment with L-arginine) and group V (melatonin injected group). All groups were subjected to measurements of serum amylase and lipase activities; total antioxidant capacity (TAC) levels, serum and tissue TNF α levels; MPO and PON1 enzymes activities, in addition to histopathological examination of all pancreatic specimens. Results: L-arginine injected group showed severe necrotizing pancreatitis confirmed by histopathological changes and significant elevations in serum amylase and lipase activities relative to the control values. Also, significant increase of serum TAC, serum and tissue TNF-α levels and MPO enzyme activity and significant decrease of PON-1 enzyme activity were detected in L-arginine injected group as compared to control group. In melatonin injected groups, either pre- or post- treatment with L-arginine, serum amylase and lipase activities and both serum and tissue TNF-α levels, MPO activity were significantly decreased in both groups with significantly lower levels in rats given melatonin pre- treatment with L-arginine. Also, significant increase of both serum and tissue PON-1 were found in both groups. Conclusion: These data might confirm the protective effect of melatonin as an antioxidant in acute pancreatitis. | ||||
| Keywords | ||||
| Acute pancreatitis (AP); tissue tumor necrosis α (TNF α); myeloperoxidase (MPO); paraoxonase-1 (PON-1) | ||||
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