Isolation, molecular identification and in vitro screening of Talaromyces purpurogenus for the first report as lemon soft- rot causal agent in Egypt
Hassan, N., Khalaphallah, R. (2024). Isolation, molecular identification and in vitro screening of Talaromyces purpurogenus for the first report as lemon soft- rot causal agent in Egypt. EKB Journal Management System, 6(2), 185-195. doi: 10.21608/svuijas.2024.284747.1362
Naglaa M Hassan; R. Khalaphallah. "Isolation, molecular identification and in vitro screening of Talaromyces purpurogenus for the first report as lemon soft- rot causal agent in Egypt". EKB Journal Management System, 6, 2, 2024, 185-195. doi: 10.21608/svuijas.2024.284747.1362
Hassan, N., Khalaphallah, R. (2024). 'Isolation, molecular identification and in vitro screening of Talaromyces purpurogenus for the first report as lemon soft- rot causal agent in Egypt', EKB Journal Management System, 6(2), pp. 185-195. doi: 10.21608/svuijas.2024.284747.1362
Hassan, N., Khalaphallah, R. Isolation, molecular identification and in vitro screening of Talaromyces purpurogenus for the first report as lemon soft- rot causal agent in Egypt. EKB Journal Management System, 2024; 6(2): 185-195. doi: 10.21608/svuijas.2024.284747.1362

Isolation, molecular identification and in vitro screening of Talaromyces purpurogenus for the first report as lemon soft- rot causal agent in Egypt

SVU-International Journal of Agricultural Sciences
Volume 6, Issue 2, April 2024, Page 185-195  XML PDF (473.87 K)
Document Type: Original Article
DOI: 10.21608/svuijas.2024.284747.1362
View on SCiNiTO View on SCiNiTO
Authors
Naglaa M Hassan email 1; R. Khalaphallahorcid 2
1Department of Agricultural Botany (Plant pathology), Faculty of Agriculture, South Valley University, 83523 Qena, Egypt.
2Department of Agricultural Botany (Microbiology), Faculty of Agriculture, South Valley University, 83523 Qena, Egypt.
Abstract
lemon (Citrus limon L.) is one of the fruit crops that is most widely cultivated worldwide.  Seven isolates of Penicillium sp were isolated from infected lemon fruits with soft rot pathogen collected from different lemon farm’s grown under Qena governorate, Upper-Egypt. Pathogenicity test under laboratory conditions reveled that all isolates caused lemon soft rot with different degree. Isolate(Q-66) was the most aggressive one. Morphological identification appeared that the fungus related to Talaromyces species. Molecular analysis confirmed the fungus had 99.47% to 100% similarity of DNA with Talaromyces purpureogenus. Effect of Trichoderma harzianum, Saccharomyces cerevisiae and Candida oleophila for inhibit the soft rot pathogen growth linear in vitro was tested, the most significant reduction in the linear growth of the fungus was achieved by Trichoderma harzianum (88.3%) followed by Saccharomyces cerevisiae (80.4%), and Candida oleophila had (76%). For our knowledge this is the first report of Talaromyces purpurogenus fungus isolation in Egypt as lemon fruits soft rot causal agent.
Keywords
lemon soft rot; biocontrol; Talaromyces purpurogenus; Trichoderma harzianum; Saccharomyces cerevisiae; Candida oleophila
Statistics
Article View: 202
PDF Download: 220