Efflux pump and extended spectrum beta-lactamases as virulence determinants in Multi Drug Resistant Klebsiella pneumoniae isolated from patients with respiratory tract infection | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Volume 33, Issue 3, July 2024, Page 49-56 PDF (341 K) | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2024.295892.1258 | ||||
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| Authors | ||||
Shimaa E.E. Siam  ; Amr M. El-Sabbagh
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| Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt | ||||
| Abstract | ||||
| Background: Antibiotic resistance is a real life threatening problem. Klebsiella pneumoniae (K.pneumoniae) is an important pathogen which is usually associated with respiratory tract infection. ESBL and efflux pump are two major causes of antibiotic resistance in K.pneumoniae. Objectives: The aims of this study were to determine the prevalence of multidrug resistant (MDR) K.pneumoniae strains among respiratory tract infection patients in Chest Department, Mansoura University Hospital (MUH) and to evaluate the presence of CTX-M ESBL and AcrAB efflux pump in these strains. Methodolgy: The study was conducted on 50 K.pneumoniae isolates from respiratory tract infected patients which were subjected to antimicrobial susceptibility testing to determine the MDR isolates that were phenotypically tested for presence of ESBL production and efflux pump mediated antibiotic resistance by double disk synergy test and microdilution test respectively followed by detection of CTX-M and Acr-A genes by PCR in the phenotypically positive isolates. Results: Antibiotic sensitivity testing of the 50 K.pneumoniae isolates revealed that all of them (100%) were MDR, of which 8 (16.0%) were positive for ESBL production and 33 (86.8%) were positive for efflux pump system by phenotypic tests. PCR revealed that CTX-M gene was founed to be harbored by 4 (8.0%) in those MDR strains and Acr A gene was detected in 33(86.8%) in the phenotypically positive strains. Conclusion: AcrAB efflux pump was more prevalent in MDR K.pneumoniae strains than CTX-M ESBL; this may be helpful in improving the health outcome during designation of the patient treatment regimen. | ||||
| Keywords | ||||
| MDR K.pneumoniae; CTX-M; AcrAB efflux pump | ||||
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