Acr AB and Oxq AB Efflux Pump Genes Among Resistant Klebsiella Pneumoniae Isolated from Zagazig University Hospitals
El-sayed, H., El Maghraby, H., Hussein, S., El Azawy, D., Attia, O., Mousa, B., Orabi, E., Fahmy, Y. (2024). Acr AB and Oxq AB Efflux Pump Genes Among Resistant Klebsiella Pneumoniae Isolated from Zagazig University Hospitals. EKB Journal Management System, 33(3), 67-75. doi: 10.21608/ejmm.2024.297587.1264
Hend A. El-sayed; Hanaa M. El Maghraby; Samia Hussein; Doaa Sh. El Azawy; Osama Attia; Bassam Mousa; Eman Orabi; Yasmin A. Fahmy. "Acr AB and Oxq AB Efflux Pump Genes Among Resistant Klebsiella Pneumoniae Isolated from Zagazig University Hospitals". EKB Journal Management System, 33, 3, 2024, 67-75. doi: 10.21608/ejmm.2024.297587.1264
El-sayed, H., El Maghraby, H., Hussein, S., El Azawy, D., Attia, O., Mousa, B., Orabi, E., Fahmy, Y. (2024). 'Acr AB and Oxq AB Efflux Pump Genes Among Resistant Klebsiella Pneumoniae Isolated from Zagazig University Hospitals', EKB Journal Management System, 33(3), pp. 67-75. doi: 10.21608/ejmm.2024.297587.1264
El-sayed, H., El Maghraby, H., Hussein, S., El Azawy, D., Attia, O., Mousa, B., Orabi, E., Fahmy, Y. Acr AB and Oxq AB Efflux Pump Genes Among Resistant Klebsiella Pneumoniae Isolated from Zagazig University Hospitals. EKB Journal Management System, 2024; 33(3): 67-75. doi: 10.21608/ejmm.2024.297587.1264

Acr AB and Oxq AB Efflux Pump Genes Among Resistant Klebsiella Pneumoniae Isolated from Zagazig University Hospitals

Egyptian Journal of Medical Microbiology
Volume 33, Issue 3, July 2024, Pages 67-75    PDF (464.58 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2024.297587.1264
Authors
Hend A. El-sayed* 1; Hanaa M. El Maghraby2; Samia Hussein2; Doaa Sh. El Azawy3; Osama Attia4; Bassam Mousa5; Eman Orabi6; Yasmin A. Fahmy1
1Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
2Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
3Anesthesia and Intensive Care Unit Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
4Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
5General Surgery Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
6Public Health and Community Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Abstract
Background: Overexpression of Acr AB and Oxq AB efflux pump genes in Klebsiella pneumoniae could increase its resistance to antimicrobials. Objectives: to explore the prevalence of Acr AB and Oxq AB efflux pump genes in resistant isolates of K. pneumoniae and to detect their role in conferring resistance to ciprofloxacin. Methodology:  Isolation of K. pneumoniae was followed by detection of its antimicrobial susceptibility. Then, the ciprofloxacin minimum inhibitory concentrations (MIC) were analyzed by broth microdilution method before and after treatment with the efflux pump inhibitor, carbonyl cyanide 3-chlorophenylhydrazone.  The presence of Acr AB and Oxq AB efflux pump genes was investigated by polymerase chain reaction. Results: One hundred K. pneumonia strains were isolated and 65 % of these isolates were multidrug-resistant with high resistance for sulphamethoxazole (77%), amikacin (66%), and ciprofloxacin (63%). 60.3% of ciprofloxacin-resistant K. pneumoniae had a significant 4-fold decrease or more reduction in MIC for ciprofloxacin after CCCP addition (p value<0.05).  There was a significant difference concerning the frequency of efflux pump genes in ciprofloxacin-resistant isolates as 82.5% were positive and acrA, acrB, oqxA, and oqxB were detected in 77.7, 71.4, 62 and 65% of isolates, respectively. Conclusion: Acr AB and Oxq AB efflux pump genes were prevalent among K. pneumoniae isolates and they had an essential role in explaining their resistance to ciprofloxacin.
Keywords
K. pneumoniae; efflux pump; and ciprofloxacin
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