Candiduria in Catheterized Children’s Patients: Association with UT, In-vitro Biofilm Formation, and Standardized VITEK 2 System for Isolated Candida Species | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Volume 33, Issue 3, July 2024, Page 77-85 PDF (602.64 K) | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2024.292829.1253 | ||||
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| Authors | ||||
Aya A. Husseiny  1; Mohamed A. Abou-Zeid2; Yosra M. Hassan3; Nevin A. Ibrahim4
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| 1Armed Forces Pharmaceutical Factory, Nahia Street - Boulak El Dakrour, Giza, Egypt | ||||
| 2Faculty of Science, Galala University, Galala City, Suez, Egypt; Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt | ||||
| 3Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Egypt | ||||
| 4Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt | ||||
| Abstract | ||||
| Background: Urinary tract infections (UTIs) by Candida species are becoming a common finding in hospitalized patients. Such association is higher in hospitalized pediatric patientsCandida albicans is the predominant fungal pathogen implicated in UTIs. Objective: This study aimed to demonstrate the prevalence of candiduria in pediatric patients with UTIs, characterize the Candida species involved, and evaluate their susceptibility to antifungal agents. Methodology: Candida species were isolated and identified in urine samples collected from 100 hospitalized children, comprising 76% from Inpatients’ Units and 24% from Outpatients’ Units, who were ranged in age from 25 days to 15 years. The automated VITEK 2 system was employed for both the identification of Candida species and susceptibility testing against various antifungal agents. Biofilm production from both albicans and non-albicans Candida was determined using the microtiter plate method. Results: Non-albicans Candida constituted more than half (52%) of the Candida isolates. A higher incidence of Candida species was observed in male patients from Inpatients’ Units in the 1-5 years age group. Additionally, this study revealed a greater prevalence of Candida species among pediatric patients receiving antibiotic treatment. The seven identified Candida species exhibited varying sensitivity to antifungal agents. Biofilm formation was more prevalent in non-albicans Candida. Conclusion: Candida tropicalis was the most prevalent non-albicans Candida (40%), being the most prolific biofilm producer. Amphotericin B and Micafungin were the drug of choice according to their complete sensitivity pattern. | ||||
| Keywords | ||||
| Key words Candida spp; Catheterization; Urinary tract infection; Children’s patients; Biofilm | ||||
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