Genotypic Characterization of Antibiotic Resistant Escherichia coli Isolates Recovered from Baladi Chicks in Fayoum Governorate | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 62, Issue 4, October 2016, Pages 63-70 PDF (2.71 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2016.365345 | ||
| Authors | ||
| Samia El-Kholi* 1; Hatem E2; El-Hariri M2; Zaki M3; Abeer Shehata4 | ||
| 1Directorate of Veterinary Medicine, Fayoum, Egypt | ||
| 2Microbiology Department, Faculty of Veterinary Medicine, Cairo University | ||
| 3Poultry Diseases, Faculty of Veterinary Medicine, Cairo University | ||
| 4Department of Bacteriology, Animal Health Research Institute, Fayoum Laboratory, Agricultural Research Center, Egypt | ||
| Abstract | ||
| A total of 23 isolates of E. coli, recovered from Baladi chicks manifested respiratory signs associated with diarrhea and belonged to different O-serogroups was investigated for antimicrobial resistance and tested for their tetracycline and ciprofloxacin resistance genes. The result recovered that 91.3% of the isolates were resistant to tetracycline and 26.1% to ciprofloxacin (quinolone group). Ten out of 23 E. coli isolates tested for detection of antimicrobial resistance genes, 5 (50%) isolates were positive for tetA(A) gene, where 5 (50%) isolates were positive for tetA(B) gene. On the other hand all the E. coli isolates 10 (100%) were positive for gyrA gene, where only one isolate (10%) was positive for aac(6’)-Ib-cr gene. | ||
| Keywords | ||
| Multiple drug resistant E. coli in poultry; Antimicrobial sensitivity test; PCR; Detection of the resistant genes of E. coli | ||
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