EVALUATION OF BONE HEALING BY MESENCHYMAL STEM CELLS IN RABBITS (AN EXPERIMENTAL STUDY) | ||
| Alexandria Dental Journal | ||
| Article 4, Volume 50, Issue 2, August 2025, Pages 24-30 PDF (473.6 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/adjalexu.2024.246336.1431 | ||
| Authors | ||
| Fadi Fouad Salib* 1; Magda Mohamed Saleh2; Radwa Mehanna3; Marwa Morsy Essawy4; Lydia Nabil Melek5 | ||
| 1Oral and Maxillofacial Surgery, Oral and Maxillofacial Surgery Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt | ||
| 2oral and Maxillofacial surgery, Faculty of Dentistry Alexandria University | ||
| 3Department of Medical Physiology, Faculty of Medicine, Alexandria University, Egypt Center of Excellence for Research in Regenerative Medicine and Applications (CERMA), Faculty of Medicine, Alexandria University, Alexandria, Egypt | ||
| 4Oral Pathology Department, Faculty of Dentistry, Alexandria University, Egypt. Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Egypt | ||
| 5Associate Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Alexandria University, Egypt | ||
| Abstract | ||
| Background: Large bone defects are considered a massive challenge in the field of oral and maxillofacial surgery. Cell Therapy using bone marrow-derived mesenchymal stem cells (BM-MSCs), as an alternative technique, has effective potential for bone regeneration. Aim of the study: To compare histologically the bone healing rate of critical size defects in rabbit tibia using mesenchymal stem cells versus untreated defects. Materials and Methods: Critical-sized defects were prepared on the tibia of rabbits. Experimental groups were divided into 2 groups: (a) Control group; untreated rabbits left for spontaneous healing, (b) collagen sponge with MSCs treated group. Sacrificing of rabbits was done at 2 and 6-week intervals. Results :Bone defects treated with BM-MSCs showed a marked increase in the healing rate compared to the control group. Histological examination showed immature bone trabeculae lined with plump active osteoblasts with minimal amount of newly formed blood vessels and almost no inflammatory cell infiltration at the 2-week interval. Furthermore, at the 6-weeks interval the defects were almost healed with mature thickened dense bone trabeculae lined by fattened osteoblasts with no inflammatory infiltration. Conclusion: BM-MSCs are a promissing tool for bone regeneration. | ||
| Keywords | ||
| Mesenchymal stem cells; Bone healing; Rabbits; Critical size defect | ||
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