Characterization of aminoglycoside-resistant Enterobacteriaceae isolated from inanimate hospital surfaces in Egypt

Fahmy, Amr H.; El-Dogdog, Khaled A.; Abd-Elsalam, El-Sayed T.; Salah, Mohamed G.

doi:10.21608/ejmm.2024.299848.1269

	Characterization of aminoglycoside-resistant Enterobacteriaceae isolated from inanimate hospital surfaces in Egypt
Egyptian Journal of Medical Microbiology
Volume 33, Issue 3, July 2024, Page 119-124 PDF (412.02 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2024.299848.1269
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Authors
Amr H. Fahmy¹; Khaled A. El-Dogdog²; El-Sayed T. Abd-Elsalam¹; Mohamed G. Salah ¹
¹Botany and Microbiology Department, Faculty of Science, Cairo University, 12613 Giza, Egypt
²Microbiology Department, Faculty of Agriculture, Ain Shams University
Abstract
*Background:* The emergence of antibiotic resistance is one of the major issues facing global healthcare systems. Objectives: This study aims to determine the prevalence and resistance profiles of Enterobacteriaceae isolated from inanimate surfaces of an Egyptian hospital. Methodology: MALDI-TOF identified Gram-negative bacteria and the antibiotic resistance profile of Enterobacteriaceae isolates was determined by Vitek 2 system. Results: From the inanimate surfaces, 266 isolates of Gram-negative nosocomial bacteria were identified of which 175 (65.79%) belonged to Enterobacteriaceae. The most frequently isolated bacteria were Klebsiella pneumoniae (n=72), followed by Escherichia coli (n=65) while Acinetobacter baumannii (n=54) and Pseudomonas aeruginosa (n=21) were the dominant non-Enterobacteriaceae isolates. Moreover, the Enterobacteriaceae isolates exhibited high degrees of resistance against aminoglycosides, penicillins, and carbapenem. In addition, various aminoglycoside-resistance genes were detected by the polymerase chain reaction (PCR). Results revealed that most Enterobacteriaceae isolates harbours aac(3′)-Ib gene (89.1%) followed by aph(3′)-Ia (52.5%) and aac(3)-II (50.2%). Conclusion: Our findings demonstrate that inanimate surfaces may be potential reservoirs of resistant Gram-negative bacteria, which directly threaten hospitalized patients.
Keywords
Aminoglycoside resistance; Inanimate surface; Enterobacteriaceae prevalence, Nosocomial infection


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