Detection of Helicobacter pylori infection by three invasive methods in gastric disorders patients from Mosul City /Iraq | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 12 July 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2024.298951.2027 | ||||
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| Authors | ||||
Muhammad Abdul-Ghani Muhammad   ; Anmar Ahmed Altaie ; Basima Ahmed Abdullah
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| Department of Biology, College of Science, University of Mosul, Mosul, Iraq | ||||
| Abstract | ||||
| Background: The identification of H. pylori in Iraq lacks a standardized technique and there is limited information on the efficacy of different diagnostic methods. Objectives: The research aims to determine the prevalence of H. pylori and evaluate the effectiveness of invasive techniques. Method: Gastric biopsy specimens were collected from 50 patients with gastric disorders to detect H. pylori infection by Rapid Urease Test (RUT), histopathological examination, and ure A gene detection by PCR. Patients were classified into H. pylori -positive cases and negative cases by testing positive with at least two of the three testing methods, according to the case definition used in the study. Result: Out of the 50 cases, 58% were H. pylori -positive cases, 26% were H. pylori -negative cases, and the last 16% were indeterminate cases according to cases definition. Females and older age individuals had the highest prevalence of H. pylori infection. The ure A gene detection by PCR was the most sensitive method, followed by histopathological examination and RUT testing, with sensitivities of 96.6%, 86.2% and 79.3% respectively. Histopathology examination was the most specific with a specificity of 95.2%, followed by ure A gene PCR with 85.7%, while RUT was the least specific with a specificity of 81%. Conclusion: The current study revealed a moderate prevalence of H. pylori infection compared with previous studies. the sensitivity and specificity of the three invasive methods varied, and a combination of two methods may be necessary for a definitive diagnosis of H. pylori infection. | ||||
| Keywords | ||||
| H. pylori; Gastric Disorders Patients; Invasive methods | ||||
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