The Beneficial Role of Magnetized Water on Methotrexate-Induced Acute Kidney Injury in Albino Rats | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 68, Issue 3, March 2025, Page 653-668 PDF (1.29 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2024.290311.9734 | ||||
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| Authors | ||||
Hanaa H. Ahmed 1; Soheir Kotob  2; Mahmoud Hozayn3; Alaa H. Sayed4; Ola A. Mohawed 4
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| 1Hormones Dept., Medical Research Division, National Research Centre | ||||
| 2Hormones Department, Medicine and Clinical Studies Research Institute, National Research Centre, Dokki, Giza, Egypt | ||||
| 33Department of Field Crop Research, Agricultural and Biological Research Institute, National Research Centre, Dokki, Giza, Egypt | ||||
| 4Hormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Giza, Egypt | ||||
| Abstract | ||||
| This research was initiated to reveal the beneficial properties of magnetized water (MW), alone or in combination with the angiotensin-converting enzyme inhibitor, Lisinopril, (Lis) on methotrexate (MTX)-induced AKI in rats, and to track the mechanisms involved in this impact. The results showed that the treatment with MW and Lis, either separately or in combination, brought about a significant reduction in serum urea and creatinine levels that were elevated upon administration with MTX. Both of MW and Lis inhibited the oxidative stress burden caused by MTX as indicated by lowering nitric oxide levels and up-regulating the total antioxidant capacity. Furthermore, both treatments decreased the increased levels of serum NAG, MIP-2 and KIM-1, which were enhanced due to MTX administration. Immunohistochemical examination of kidney tissue of both of MW- and Lis-treated groups reveal moderate and mild immune reactions for Cas-3, TNF-α, TGF-β and Bax, respectively, while the combined treatment resulted in negative immune reaction. The biochemical and immunohistochemical findings were confirmed by the histopathological investigation where both of MW and Lis exhibited moderate ameliorative effects on kidney tissue while the co-treatment produced marked improvement in the histological feature of kidney tissue. In conclusion, the co-administration of MW and Lis can synergistically attenuate the MTX-induced renal injury. Drinking MW as an adjuvant agent with Lis during the treatment of AKI can enhance the efficiency of this drug. This could be attributed to the advantage of MW as an antioxidant, anti-inflammatory and anti-apoptotic candidate. | ||||
| Keywords | ||||
| Acute kidney injury; Methotrexate; Magnetized water - Angiotensin-converting enzyme inhibitor; Inflammation; Oxidative stress; Apoptosis; Rats | ||||
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