PHENOTYPIC AND GENETIC CHARACTERIZATION OF PASTEURELLA MULTOCIDA WITH A REFERENCE TO ITS PATHOLOGICAL CHANGES IN CALVES | ||
| Assiut Veterinary Medical Journal | ||
| Article 2, Volume 70, Issue 183, October 2024, Pages 11-24 PDF (1.72 M) | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.2024.272597.1229 | ||
| Authors | ||
| NESMA RASHEED1; HALA M. ISMAIL2; MAHMOUD A. ABDELRAHMAN3; MARWA SHALABY* 3 | ||
| 1Department of Pathology, Animal Health Research Institute (AHRI), Mansoura Branch, Agriculture Research Center (ARC), P.O. Box 264 Dokki, 12618 – Giza, Egypt. | ||
| 2Department of Pathology, Animal Health Research Institute (AHRI), Mansoura Branch, Agriculture Research Center (ARC), P.O. Box 264 Dokki, 12618 – Giza, Egypt | ||
| 3Department of Bacteriology, Animal Health Research Institute (AHRI) (Mansoura Branch), Agriculture Research Center (ARC), P.O. Box 264 Dokki, 12618 – Giza, Egypt. | ||
| Abstract | ||
| This study investigated the presence of Pasteurella multocida in Egyptian dairy farm calves with respiratory symptoms. A total of 150 samples were collected aseptically from 133 nasal swabs and 17 pneumonic lung tissues of calves on private farms across different governorates. Samples were tested using three methods: standard bacteriological examination, biochemical identification, and Molecular characterization for P. multocida. The P. multocida isolates were subjected to multiplex PCR for capsule biosynthesis and 16S rRNA gene. Capsular typing revealed that 60 out of 150 samples (40%) were positive for P. multocida. All isolates confirmed as P. multocida through PCR were belonged to serogroup A. Antibiotic sensitivity testing of these isolates identified enrofloxacin as the most effective drug. The most detectable resistance gene was sul II- in examined isolates with a 100% detection rate, followed by BlaTEM and tetA genes (62.5%). Histopathological examination revealed extensive damage across various organs, such as the lungs of calves, including fibrinous exudate inside alveoli and purulent fluid in other alveoli, besides thrombosis of blood vessels in P. multocida-infected animals. | ||
| Keywords | ||
| Pasturella multocida: Molecular Characterization: Serotyping: Histopathology | ||
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