Green Spectrophotometric Method for the Determination of Tigecycline in Different Matrices | ||||
| Records of Pharmaceutical and Biomedical Sciences | ||||
| Volume 8, Issue 1, 2024, Page 92-105 PDF (902.14 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/rpbs.2024.299538.1304 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Noha Ali Mansour  1; Alaa El-Gendy2; Mary Wahba3; Aziza El_Sayed Mostafa4
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| 1Analytical chemistry, faculty of pharmacy, Suez canal university, Egypt | ||||
| 2Faculty of Pharmacy - Suez Canal University | ||||
| 3Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Delta University for Science and Technology, Egypt | ||||
| 4lecturer, Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt | ||||
| Abstract | ||||
| This work presents a green, simple, validated colorimetric method for the determination of tigecycline in lyophilized powder and laboratory prepared pharmaceutical wastewater. The suggested method depends on the reaction of tigecycline with Eosin-Y targeting its tertiary amino group, forming a reddish-orange ion pair complex with a wavelength of maximum absorbance of 555 nm. The greenness of this method was assessed by four methods; 1-National Environmental Methods Index assessment (NEMI), 2-Analytical Eco-Scale assessment, 3-Green Analytical Procedure Index assessment (GAPI), and 4-The Analytical Greenness Metric assessment (AGREE). They showed the significant greenness of the proposed method. The analyte followed a linear pattern over the concentration range of 1.0-14.0 μg/mL, with a lower detection limit of 0.21 μg/mL and a quantitation limit of 0.64 μg/mL. The suggested method was completely validated according to the International Conference on Harmonization (ICH) guidelines regarding linearity, accuracy, precision, specificity, and robustness, which permits its application for the intended purpose. | ||||
| Keywords | ||||
| Tigecycline; Eosin-Y; colorimetry; wastewater | ||||
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