Identification of Lantibiotic Producing Lactic Acid Bacteria and Its Use Combined With Irradiation For Food Preservation | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 68, Issue 3, March 2025, Page 345-359 PDF (744.25 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2024.293069.9783 | ||||
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| Authors | ||||
Mai Anwar Zaki   1; Mohamed Abou-Zeid2; Samar Mohamed3; Ali Ahmed Hammad4; Salwa Abou El-Nour5
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| 1Radiation Microbiology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt | ||||
| 2Faculty of Science, Galala University Department of Microbiology, Faculty of Science, Ain Shams University | ||||
| 3Microbiology department, Faculty of Science, Ain Shams University, Cairo, Egypt. | ||||
| 4Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority | ||||
| 5Radiation Microbiology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt. | ||||
| Abstract | ||||
| The purpose of this study was to isolate lactic acid bacteria able to produce lantibiotics from dairy products containing them and evaluate their effects as food biopreservatives when combined with gamma irradiation. Twenty-five LAB isolates were isolated, among which fourteen proved to produce antibacterial substances at various levels. By using conventional methods, the two isolates that produced the highest yield were identified as Streptococcus thermophilus, and this identification was further verified by 16S ribosomal RNA (16S rRNA) sequence analysis. Lantibiotic was extracted from culture using chloroform. Liquid Chromatography Mass Spectrometry (LC/MS) and Gas chromatography–mass spectrometry (GC–MS) were used to characterize the extracted lantibiotic after it had been purified using Fast Protein Liquid Chromatography (FPLC). The resulting chromatograms indicated the presence of dehydrobutyrine amino acid, which is characteristic of lantibiotic compounds. Supernatant containing lantibiotics obtained from Streptococcus thermophilus was added to samples of minced meat and cut carrot at concentrations of 20 and 40 ml/kg, either separately or in combination with a dosage of 2 kGy gamma irradiation. Compared to the control samples, which had a storage life of only one week, these treatments effectively increased the samples' shelf life to two and three weeks at 4°C±1, respectively. To improve food preservation and microbiological safety, this study supports the use of natural biopreservatives as an alternative to chemical preservatives, either on their own or in combination with low doses of gamma irradiation. | ||||
| Keywords | ||||
| lantibiotics; lactic acid bacteria; irradiation; preservation of food | ||||
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