Estimation of the post-mortem interval using microRNA in the heart in aluminum phosphide deaths
Ahmad, M., ghaleb, S., Zaki, A., Kamel, A., Abdalla, A. (2024). Estimation of the post-mortem interval using microRNA in the heart in aluminum phosphide deaths. EKB Journal Management System, 5(3), 24-34. doi: 10.21608/ejmr.2024.263063.1529
Manar A Ahmad; Sherein S ghaleb; Amr R Zaki; Abeer O Kamel; Ahmed G. Sobhi Abdalla. "Estimation of the post-mortem interval using microRNA in the heart in aluminum phosphide deaths". EKB Journal Management System, 5, 3, 2024, 24-34. doi: 10.21608/ejmr.2024.263063.1529
Ahmad, M., ghaleb, S., Zaki, A., Kamel, A., Abdalla, A. (2024). 'Estimation of the post-mortem interval using microRNA in the heart in aluminum phosphide deaths', EKB Journal Management System, 5(3), pp. 24-34. doi: 10.21608/ejmr.2024.263063.1529
Ahmad, M., ghaleb, S., Zaki, A., Kamel, A., Abdalla, A. Estimation of the post-mortem interval using microRNA in the heart in aluminum phosphide deaths. EKB Journal Management System, 2024; 5(3): 24-34. doi: 10.21608/ejmr.2024.263063.1529

Estimation of the post-mortem interval using microRNA in the heart in aluminum phosphide deaths

Egyptian Journal of Medical Research
Volume 5, Issue 3, July 2024, Page 24-34  XML PDF (846.87 K)
Document Type: Original Article
DOI: 10.21608/ejmr.2024.263063.1529
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Authors
Manar A Ahmad1; Sherein S ghaleb2; Amr R Zaki1; Abeer O Kamel email 1; Ahmed G. Sobhi Abdalla1
1forensic medicine and clinical toxicology department, Faculty of medicine, Beni-suef university, Beni-suef
2forensic medicine and clinical toxicology ,faculty of medicine, cairo university- Egypt
Abstract
Background: The assessment of the postmortem interval (PMI) represents one of the major challenges in forensic pathology. Because of their stability miRNAs, are anticipated to be helpful in forensic research. Objective: to see if estimation of PMI is possible using miRNA-122 expression levels in the heart samples from aluminum phosphide toxicity (Alpt). Methods: This was a cross sectional study on 60 post-mortem samples (heart tissues) collected at different intervals during forensic autopsies. The two groups were allocated equally according to the cause of death into Group I (control, n꓿30): Deaths caused by other than toxicity, and Group II (cases, n꓿30): Deaths due to Alpt. miRNA- 122 expression levels were measured in heart tissues at different PMI using RT-Q PCR.  Results: miRNA-122 level in Alp deaths was up regulated with statistically significant difference. There was positive correlation between miRNA-122 with PMI. Conclusion: The results of this study concluded that the PMI can be calculated using the degree to which particular miRNA-122.
Keywords
post mortem interval; miRNA-122; heart; aluminum phosphide deaths
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