Recent isolation and identification of Equine herpes viral abortion (EHV-1) in Egypt-2007 | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 57, Issue 4, October 2009, Page 811-820 PDF (2.76 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2011.368067 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Nehal Saleh; Eman Ebied; Magda Kalad; Soliman A | ||||
| Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo | ||||
| Abstract | ||||
| The present study was planned to isolate and identify the local Equine herpes viral abortion (EHV-1). So, samples (Liver, lung, spleen, placenta and serum) of 5 aborted mares and their foeti were collected from a private stud in Egypt, with a history of recurrent abortion during (2005, 2006). Attempts for isolation of the causative agent were performed on chorioallantoic membrane of SPF embryonated chicken eggs and tissue culture (VERO cells). The highest titre was obtained from the liver of foeti which ranged from 8-9 log10 EID50 at 3rd egg passage and 7-8 log10 TCID50 at 5th tissue culture passage. These results were confirmed by different serological tests (AGPT, VN test, ELISA and IF) by using reference rabbit anti-EHV-1 sera. In addition, antibodies against EHV-1 were detected in mares sera by AGPT and ELISA. | ||||
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