Trials for improving the efficiency of inactivated Newcastle disease virus vaccine by using propioni-bacterium and lipopolysaccharide (IM-104) as immunostimulant | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 57, Issue 4, October 2009, Page 821-828 PDF (2.19 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2011.368068 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Hala El-Makaky | ||||
| Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo | ||||
| Abstract | ||||
| The ability to improve the efficacy of an inactivated Newcastle disease vaccine by addition of immunomodulator as propioni- bacterium and lipopolysaccharide (IM-104) was studied. Chickens were divided into three groups, one received inactivated Newcastle vaccine containing the IM-104, second group received vaccine alone and third group of chickens was left without vaccination as control. Blood samples were collected 3 days post vaccination, and weekly till four weeks for estimation of the effect of (IM-104) on T-lymphocyte transformation (cellular immune response). Sero-conversion for sera at weekly intervals was done to evaluate the immune response using HI test and ELISA. Challenge test was carried out on 3rd and 6th week post vaccination for evaluation of protective efficacy of these vaccines. The results obtained revealed that (IM-104) that contain 2 extraction of E. coli lipopolysaccharide and propioni-bacterium plays roles in stimulating both T-lymphocyte and macrophage to release different types of interleukin and consequently released immunoglobulins. Also, stimulate non-specific resistance to viral infection. The results obtained by challenge test run in parallel with that of serological test. | ||||
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