Preparation and Evaluation of a Combined Inactivated Oil Emulsion Vaccine against Newcastle and Chicken Necrotic Enteritis Diseases | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 59, Issue 3, July 2011, Page 65-72 PDF (1.32 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2011.368231 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Salman A* 1; Hanaa Mostafa2; Ghada Sadek2; Hussein S1; Elham El-Ebiary2; Khodeir H1; Makharita A2 | ||||
| 1Veterinary Serum and Vaccine Research Institute; Abassia,Cairo | ||||
| 2Central Laboratory for Evaluation of Veterinary Biologics | ||||
| Abstract | ||||
| The present work represents a trial for preparation and evaluation of a combined vaccine against Newcastle disease (ND) and necrotic enteritis (NE) as a viral and bacterial diseases affecting poultry industry in a dramatic form. The obtained results showed that the prepared vaccine was stable; free from foreign contaminants; safe and immunogenic for vaccinated chickens. The comparison between the efficacy of single ND and NE vaccines with the efficacy of the prepared combined ND and NE vaccine revealed that both vaccines were Immunogenic inducing protection against the challenge with virulent strain of ND virus of 87 and 93% in single and combined vaccines respectively. Non vaccinated chickens were unable to withstand the virulent strain of ND virus. Haemagglutination inhibition test (HI) for estimation of ND antibodies was confirmed by the results of challenge test. It was noticed that the combined vaccine induced higher antibody titer and higher protection than those induced by single vaccine. On the other hand, using serum neutralization test for estimation of the antibodies against NE revealed that the antibody titer was higher in combined vaccine than single vaccine. So, it could be suggested that the combined ND and NE vaccine is preferable than the single vaccines. | ||||
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