Preparation of monospecific antisera against fowl cholera, fowl coryza and Newcastle disease reference strains in laboratory animals
El-Sadek, G., El-Sawah, A., Mostafa, H., Mahmoud, H., M, E., El-Ebiary, E., H, A. (2011). Preparation of monospecific antisera against fowl cholera, fowl coryza and Newcastle disease reference strains in laboratory animals. EKB Journal Management System, 59(3), 109-116. doi: 10.21608/vmjg.2011.368235
Ghada El-Sadek; Amal El-Sawah; Hanaa Mostafa; Hanan Mahmoud; El-Safty M; Elham El-Ebiary; Abdel Baky H. "Preparation of monospecific antisera against fowl cholera, fowl coryza and Newcastle disease reference strains in laboratory animals". EKB Journal Management System, 59, 3, 2011, 109-116. doi: 10.21608/vmjg.2011.368235
El-Sadek, G., El-Sawah, A., Mostafa, H., Mahmoud, H., M, E., El-Ebiary, E., H, A. (2011). 'Preparation of monospecific antisera against fowl cholera, fowl coryza and Newcastle disease reference strains in laboratory animals', EKB Journal Management System, 59(3), pp. 109-116. doi: 10.21608/vmjg.2011.368235
El-Sadek, G., El-Sawah, A., Mostafa, H., Mahmoud, H., M, E., El-Ebiary, E., H, A. Preparation of monospecific antisera against fowl cholera, fowl coryza and Newcastle disease reference strains in laboratory animals. EKB Journal Management System, 2011; 59(3): 109-116. doi: 10.21608/vmjg.2011.368235

Preparation of monospecific antisera against fowl cholera, fowl coryza and Newcastle disease reference strains in laboratory animals

Veterinary Medical Journal (Giza)
Volume 59, Issue 3, July 2011, Page 109-116  XML PDF (1.16 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2011.368235
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Authors
Ghada El-Sadek* ; Amal El-Sawah; Hanaa Mostafa; Hanan Mahmoud; El-Safty M; Elham El-Ebiary; Abdel Baky H
Central Laboratory for Evaluation of Veterinary biologics, Abbasia, Cairo
Abstract
 Monospecific antisera against Paneurella multocida strains that belonged to capralar and somatic serogroups A:1, A:3 and A:4, Avibacterium paragallinarum serovars A and C and Newcastle disease virus, were prepared in three separate rabbit groups for each using reference bacterial and viral cultures emulsified in Freund's adjuvant. Antisera of P. multocida were qualitatively and quantitatively evaluated by serological tests using their homologous and heterologous antigens (cross matching). Sera of immunized rabbits showed a reasonable titre of P. multocida capsular group (A) antibodies by passive haemagglutination test and to somatic serotype (A) 1, 3 and 4, antibodies by tube Aghaination and agar gel diffusion tests. Serovars A and C antibodies of Avibacterium paragallinarum were detected in sera of the immunized rabbits by plate agglutination test. The main titre of 8.5 log2 haemagglutination inhibitory antibodies against Newcastle disease virus was achieved in sera of immunized rabbit which received 3 successive inoculation of ND LaSota strain. Conclusively, the attained results proved that the prepared mono-specific antisera could be used as a highly reliable guide in the serological identity tests of the challenge and vaccinal strains of fowl cholera, infectious coryza and Newcastle disease.
 
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