Molecular characterization of Mycoplasma gallisepticum isolated from Chicken and Turkey | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 59, Issue 3, July 2011, Page 183-195 PDF (5.13 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2011.368245 | ||||
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| Authors | ||||
| Eissa I* 1; Hassan M1; Metwally M2; Hashem M1; Abd El-Aziz E1 | ||||
| 1Animal Health Research Institute, Dokki- Giza-Egypt. | ||||
| 2Cairo Poultry Company, Giza, Egypt | ||||
| Abstract | ||||
| Mycoplasma gallisepticum (MG) infection in chicken and turkey is still one of the important reasons causing economic losses in poultry. The current study concerned with rapid detection and molecular characterization of MG isolates. The all samples positive by culture were positive by PCR and rt- PCR. Five isolates (four from chicken and one from turkey) were sequenced for mgc2 gene. The present molecular study proved that four wild-type MG strains. (Eis 3- C-10, Eis 4- C-10, Eis 5- C-10 were recovered from chicken and one (Eis 6- T-10) was recovered from turkey. While Eis 7-C-10 (vaccinal F- strain) was isolated from commercial layer flock vaccinated with F- strain vaccine. We concluded that mgc2 gene was able to distinguish between MG wild - type and vaccinal strains. | ||||
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