Sero-diagnostic efficacy of antigens prepared from B. abortus and B. melitensis used for agglutination tests and IELISA | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 59, Issue 3, July 2011, Pages 219-233 PDF (5.14 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2011.368248 | ||
| Authors | ||
| Mehanna A* 1; Sadek A1; Shell A2 | ||
| 1Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo (VSVRI) | ||
| 2Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo (CLEVB) | ||
| Abstract | ||
| In this study, 120 bovine and ovine serum samples were collected and tested for brucellosis with Rose Bengal test (RBT), modified Rose Bengal test (mRBT), buffered acidified plate test (BAPT) using conventional Rose Bengal antigen (RBA) and buffered acidified plate antigen (BAPA) and antigens prepared from B. melitensis (the main cause of brucllosis in Egypt) strain 16- M. Indirect ELISA was used for testing the same serum samples using 4 different coating antigens which were S-LPS and OMP antigens prepared from 16-M strain and S- LPS and OMP prepared from S19 strain. There were some differences between the results of conventional Rose Bengal and BAPA antigens and Rose Bengal and BAPA antigens prepared from B. melitensis 16-M strain. In the same time, there were no characteristic differences in results of Indirect ELISA | ||
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