Molecular profile of Staphylococcus aureus related with UTIs pregnant women in Al-Nasiriyah City, Iraq | ||||
| Microbes and Infectious Diseases | ||||
| Volume 6, Issue 3, August 2025, Page 3020-3027 PDF (1.05 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2024.297550.1999 | ||||
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| Authors | ||||
Hussain Saleh Farhood   1; Saad Salman Hamim 2
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| 1Laboratory Specialist, Ministry of Health, Thi-Qar Health Directorate, Bint Al-Huda Hospital, Thi-Qar, Iraq | ||||
| 2Department of Pathological Analysis, College of Science, University of Thi-Qar, Thi-Qar, Iraq | ||||
| Abstract | ||||
| Background: Staphylococcus aureus (S. aureus) is a facultative anaerobic, Gram-positive (GP), catalase-positive, non-motile bacterium that infects humans and animals, causing illness in a wide range of individuals as an opportunistic organism in various parts of the body, including urinary tract. The purpose of the current study was to clarify the potential impact of bacterial infection on pregnant patients at Bint Al-Huda Teaching Hospital in Thi-Qar Governorate during the period from August 2023 to January 2024. Methods: All patients provided 40 blood samples, which were then used to perform a conventional Polymerase Chain Reaction (PCR) test utilizing the universal gene. Seven S. aureus PCR products were selected and subjected to partial DNA sequencing for the 16S rRNA gene to follow up their possible relationship among what was recorded globally in GenBank. Results: The results revealed that 7/40(17.5%) isolates were S. aureus according to 16S rRNA were recorded globally in GenBank under the official accession numbers of PP396125, PP396121, PP396120, PP396122, PP396124, PP396126, and PP396123. The phylogenetic tree that was constructed by MEGA-10 software showed that there were different molecular relationships among the local S. aureus isolated from urinary tract infections (UTIs) pregnant women with analogous ones around the world. Conclusion: Pregnant women's UTIs remain one of the most common socio-health issues that require extensive diagnosis and care. When it comes to accurately diagnosing related illnesses, molecular approaches to bacterial identification in the blood stream may be helpful with faster and with high accuracyز | ||||
| Keywords | ||||
| S. aureus; UTIs; Gene sequencing; phylogenic tree | ||||
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