Reveal of Antimicrobial Effect of Lawsonia inermis Extract on Staphylococcus aureus Using Molecular and Biochemical Techniques | ||||
| Journal of Basic and Environmental Sciences | ||||
| Volume 1, Issue 1, January 2014, Page 13-23 PDF (384.79 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbes.2014.368755 | ||||
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| Authors | ||||
| 1. Abulyazid,; G. Singer; Ragaa M. Ahmed* | ||||
| Abstract | ||||
| Leaf of Lawsonia inermis (Li) was examined to their antimicrobial potential. Broth extracts in different concentrations were prepared and bioassayed in vitro for the growth of Staphylococcus aureus. It was found that the growth of Staphylococcus aureus pathogen was decreased. The protein pattern has one common band of Rf 0.24 and one characteristic band of Rf 0.27 for Staphylococcus aureus sample. Small quantitative mutation was observed in the bacteria with 5, 10, 15% of Lawsonia inermis compared with the control. Some types of proteins in Staphylococcus aureus were completely disappeared after inoculated with 5, 10, 15% Li. The catalase pattern showed one common band at R1 with Rf 0.11 and the characteristic bands observed at R2 , R3 , R4 , R5 with Rf ranged between 0.22- 0.41 for Staphylococcus aureus sample. These results confirmed the antibacterial activity of henna leaves and supported the traditional use of the plant in therapy of bacterial infections. The broth extract of the Lawsonia inermis leaves showed obvious antibacterial activity against Staphylococcus aureus. | ||||
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