Isolation and identification of Campylobacter species in apparently healthy Ostriches | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 60, Issue 2, April 2014, Pages 43-50 PDF (2.84 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2014.368829 | ||
| Authors | ||
| Heba Mohammad* 1; Jakeen El-Jakee2; Zakia A3; Mona Sobhy4 | ||
| 1Graduate student at Microbiology, Faculty of Vet. Med. Cairo Uni. | ||
| 2Prof. of Microbiology dept., Fac.of Vet. Med. Cairo Uni. | ||
| 3Prof. and headdept. of Vet hygiene& mangement. Fac.of Vet. Med. Cairo Uni. | ||
| 4Dept. of reproductive diseases. ARRI. ARC Giza, Egypt. | ||
| Abstract | ||
| In the present work, a total of 150 samples was collected from apparently healthy ostriches (102 fecal samples, 36 water samples and 12 food samples) at different geographical sites. Ostrich's samples were obtained from the zoo (46 samples),private farms (77 samples) and 27 samples from governmental farms. All samples were cultured ontothioglycolate broth at 37°C under microaerophilic condition (5% O2, 10% Co2 and 85% N2) and examined after 24 hrs under phase contrast microscope for characteristic campylobacter motility. The positive samples for isolation of Campylobacter species were identified biochemically. The rate of isolation of Campylobacterjejuni and C. coliwas 24.67 % (37out of 150 samples) as follows: where 21(14%) isolates were recovered from feces, 13(8.67%) from water samples and 3(2%) from food samples. The prevalence of C. jejuni was higher among collected in ostrich’s samples Obtained with the rate of 18 %( 27 samples). On the other hand, C. Coliwith the rate of 6.67% (10 samples). Results showed highest rate of the isolated Campylobacter species from samples obtained from the zoo18(39.13%); followed by private farms 17 (22.08%) while it was 2(7.41%) at the governmental farms. In conclusion, the isolation of Campylobacter species from ostriches gets useful information upon the diagnosis of bacterial diseases affecting these birds. Also, proper management should be offer for zoo birds to maintain their good health and hygienic programs must be applied. Preventive medical programs should be adaptive to the bird especially in the zoo, periodic fecal examinations and treatments of diseased ostrich, good nutrition and potential health care must be taken for these birds. | ||
| Keywords | ||
| Campylobacter jejuni; Campylobacter coli; ostriches; isolation; PCR | ||
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