Evaluation of Lateral Flow Immunoassay kits for diagnosis of bovine tuberculosis | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 60, Issue 3, July 2014, Page 87-96 PDF (4.92 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2014.368845 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Nasr A  1; Makharita A2; Ereny L2; Abdelrahman M1; Shereen M1
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| 1Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt. | ||||
| 2Central Laboratory for Evaluation of Veterinary Biologic Abbasia, Cairo, Egypt. | ||||
| Abstract | ||||
| Bovine tuberculosis is an enduring contagious disease of cattle that has caused substantial losses to the global livestock industry. In this study, two types of rapid tests (IQRT and Vet-TB-STAT-PAK™) have been evaluated using serum samples collected from positive reactor animals to tuberculin test. bovis was isolated from those animals. Four thousands two hundred and fifty (4250) dairy cattle were tested by single intradermal comparative tuberculin skin test, 74 (1.7%) animals reacted positively, 51 (68.9%) of the slaughtered animals showed visible lesions on post mortem (PM) examination while the other 23 (31.1%) showed non visible lesions. The bacteriological examination of the 74 samples revealed M. bovis from 44 (59.5%) of processed samples. The use of the IQRT that employed recombinant M. bovis MPB70 antigen as capture and detector, revealed that this anti M. bovis antibody test kit has detected 24.3% of tuberculin positive cattle against those confirmed by the bacteriological examination (59.9%) as M. bovis positive. On the other hand, using the other lateral flow assay Vet-TB-STAT-PAK™ has detected 36.5 % of tuberculin positive cattle against 59.5% of them confirmed by bacterial isolation as M. bovis positive. Applying the IQRT on milk samples of tuberculin reactor cattle has detected 35% of tuberculin positive cattle against 7.5% of them confirmed by bacterial isolation from milk samples as M. bovis positive while, the other lateral flow assay Vet-TB-STAT-PAK™ has detected 47.5 % of tuberculin positive cattle against 7.5% of them confirmed by bacterial isolation from milk as M. bovis positive. It is recommended that bovine tuberculosis Antibody Rapid Test Kit alone may not be reliable for screening cattle infected with tuberculosis especially in the developing countries as additional test is required to validate its results. | ||||
| Keywords | ||||
| Diagnosis; Evaluation; Lateral flow; Mycobacterium bovis | ||||
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