Correlation between Gene expression of Long Non-coding RNA (XLOC_I2_006631& ENST00000425150) and Central Obesity Phenotype in Hashimoto's Thyroiditis Patients. | ||
| Egyptian Journal of Chemistry | ||
| Volume 67, Issue 13, December 2024, Pages 759-770 PDF (583.56 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2024.294784.9795 | ||
| Authors | ||
| Thoria Ahmed Omar1; Ibrahim El Tantawy El Sayed* 2; Shimaa Kamal El Dine Zewain3; Ashwak Nasim Ibrahim Elhalafawy2; Walaa Saad Hanafy Mahmoud4 | ||
| 1Clinical Pathology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Egypt | ||
| 2Department of Chemistry, Faculty of Science, Menoufia University, Shebin El-Kom 32512, Menoufia, Egypt; | ||
| 3Internal Medicine Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Egypt. | ||
| 4Biological anthropology department, institute of medical research and clinical studies, National Research Centre, NRC, Egypt. | ||
| Abstract | ||
| Background: Hashimoto thyroiditis (HT) is the commonly occurring autoimmune disorders. It arises due to genetic predisposition, X chromosomal inactivation patterns influenced by environmental factors, and microbiome composition, resulting in an imbalance in self-tolerance mechanisms. Long non-coding RNAs (LncRNAs) are a novel family that attracted attention. It controls genes expression via alteration of chromosome conformation, variation of transcription, splicing, stability and accessibility of mRNAs, and post-translational alteration. LncRNAs have been associated with pathophysiology of various autoimmune and inflammatory diseases such as HT. Obesity and hypothyroidism are two frequently occurring clinical disorders that have been tightly connected. A novel viewpoint suggests that alterations in thyroid-stimulating hormone (TSH) may be related to obesity rather than thyroid disease. Purpose of the study: This study aimed to evaluate the expression of two lncRNAs (XLOC_I2_006631 and ENST00000425150) in peripheral blood of patients with HT, as well as their correlation with central obesity and laboratory characteristics. Method: Expression levels of lncRNAs (XLOC_I2_006631 and ENST00000425150) were determined by quantitative real-time reverse transcription polymerase chain reaction from 114 HT patients and 114 age and gender matched healthy individual serve as controls. Results: lncRNA XLOC_I2_006631 expression level was statistically significant increase in HT patient (P < 0.001). While lncRNA ENST00000425150 expression level was statistically significant decrease in HT patients (P < 0.001). Two lncRNAs were correlated significantly with serum levels of TgAb, TPOAb, waist circumference and body mass index as central obesity phenotype vital sign. Conclusion: The expressions levels of XLOC_I2_006631 and ENST00000425150 were significant, and correlate with central obesity phenotype and laboratory characteristics in in HT patients. Suggesting that these lncRNAs could be a potential biomarker for HT. | ||
| Keywords | ||
| gene expression; Hashimoto thyroiditis; Long noncoding RNAs; Real time-PCR; Obesity | ||
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