BIOLOGICAL AND IMMUNOLOGICAL CHARACTERIZATION OF ESCHERICHIA COLI PILI ISOLATED FROM CASES OF URINARY TRACT INFECTION IN HORSES | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 50, Issue 3, July 2002, Page 377-388 PDF (3.05 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2002.369260 | ||||
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| Authors | ||||
| ANSAM SALMAN; R SELEIM* ; SAHAR SAHAR; HODA ZAKI | ||||
| Bacteriology Department, Animal Health Research Institute, Doki, Giza | ||||
| Abstract | ||||
| The bacteriological examination of 126 urine samples from horses with urinary tract infection revealed the isolation of E. coli from 76 cases (60.3%); Streptococcus spp. 14 cases (11.1%); Klebsiella spp. 12 cases (9.5%); Proteus spp. 11 cases (8.7%); Staphylosoccus spp. 7 cases (5.6%) Corynebacterium 3 cases (2.3%) and 3 cases were bacteriologically negative (2.3%). The incidence of E. coli isolation from mares was higher (63.9%) than stallions (55.6%). Three E. coli serogroups were identified 0:16, (26 isolates 20.6%), 0:2, (20 isolates 15.9%), and 0:6 (16 isolates 12.7%). The SDS-PAGE analysis of the pur- ified pili preparations from each serogroup revealed one distinct protein band 20.1kDa for 0:16 and 0:6, whereas a 16.6kDa band serogroup for the serogroup 0:2. The hemagglutintion activity of E. coli isolates to the different erythrocyte types was similar to their corresponding purified pili preparations. The effect of D-mannose on the hemagglutination of different erythrocytes revealed two types of hem- agglutination, the mannose sensitive (pili type I) and the mannose resistant hemagglutination (pili type II). In the in-vitro adherence test of E. coli isolates to urinary tract epithelial cells (UTEC), all E. coli isolates were highly adherent, especially the isolates of the serogroup 0:16. In the adhesion inhibition test using the homologus antipili antisera. The number of E. coli adhered to UTEC was significantly (P<0.01) reduce, whereas the hetrologus antibodies could reduce the adhesion in case of isolates of the serogroup 0:16 and 0:6 only. While heterologus antipili antisera could not inhibit the adhesion of the isolates from the | ||||
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