AN APPROACH FOR IMPROVEMENT OF THE FOWL CHOLERA ADJUVANTED VACCINE IN EGYPT PRIVATE | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 52, Issue 4, October 2004, Page 561-573 PDF (4.97 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2004.369316 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| NAHED KHAMIS* ; A EL-KHOLY; A DAOUD | ||||
| Veterinary Serum and Vaccine Researches Institute, Abbasia, Cairo, Egypt. | ||||
| Abstract | ||||
| Reformulation of the local oil adjuvanted fowl cholera inactivated vaccine components, the nature of adjuvant and vaccinal antigen, was approached aiming to improve its protective efficacy in chickens. A substantial value of reformulation was verified by protection against challenge exposure to both virulent serotypes A and D of P: multocida and seroconversion was detected by indirect ELISA and indirect haemagglutination (IHA) assays. All vaccinal antigens comprised formalized cultures of P. multocida serotypes A:5, 8, 9 and D:2 emulsified in the currently used water-in-oil adjuvant (Span, Paraffin and Tween-80, SPT80) and/or a commercial oil- in-water adjuvant (EMC). Besides, use of non- formalin treated lysates of P. multocida as a novel vaccinal antigen was evaluated in parallel. The oil-in-water adjuvant (EMG) was preponderant when used, with and without the currently used water-in-oil adjuvant (SPT80) in all new vaccine formulations, producing a stable, more potent, less viscous emulsion and easy injectable product. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis revealed a considerable similarity in the electrophoretic patterns of cell lysates recovered from P. multocida serotypes A:5, 8, 9 and D:2, manifested as 14-16 protein bands and the molecular mass range of about 14- 190 kilodaltons. However, a considerable reduction in number of protein bands was observed in the cell lysates of their counterparts after formal- in treatment. All new vaccine formulations used in this study are found reliable for seroconversion and protection of vaccinated chickens, with variable effectiveness. Use of non-formalin treated lysates of P. multocida as a novel vaccinal antigen emulsified in EMG as an oil-in-water ad- juvant, provided an advantageous formulation of the fowl cholera vaccine that conferred a 100% protection of chickens against virulent challenge. Moreover, it became much more safer vaccine for chickens since it contains a pharmaceutical grade oil of EMG with no formalin residues. This work initiates a potential for production of safer bacte-rial vaccines for edible animals. | ||||
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