IMPACT OF ANTIOXIDANTS ON VIABILITY OF CRYOPRESERVED STALLION SPERMATOZOA CHILLED AND CRYOPESERVED STALION SPERMATOZOA | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 52, Issue 4, October 2004, Page 613-625 PDF (4.58 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2004.369323 | ||||
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| Authors | ||||
| M WAHEED* 1; T KHALIFA2 | ||||
| 1Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University. | ||||
| 2Department of Theriogenology, Faculty of Veterinary Medicine, El-Mansoura University | ||||
| Abstract | ||||
| A series of factorially arranged six experiments were conducted to study the effects of twenty- eight antioxidants on maintenance of viability of stallion spermatozoa in a chemically defined stallion semen extender (Tris egg yolk) at 5 °C and 196°C. Sixty-nine ejaculates were collected from nineteen Arabian horses (5-21 years old). In four preliminary experiments (n=40), the appropriate concentrations of the best four antioxidants in terms of sperm viability at 5°C were selected. In the fifth experiment (n= 19), a comparison between the selected antioxidants and a mixture (cocktail) of them was studied on bases of sperm viability, sperm morphology and changes in levels of extracellular aspartate and alanine aminotransferase. The sixth experiment (n = 10) was carried out for using the best antioxidant that could improve the viability of frozen – thawed stallion spermatozoa. Results of the pre- liminary experiments revealed that four antioxidants viz., sodium pyruvate, sodium thiosulfate, bovine serum albumin and zinc chloride could induce beneficial effect on viability of stallion spermatozoa. A mixture of the previous four antioxidants was most effective for improving spermatozoal morphology and viability at 5°C. Extracellular level of alanine aminotransferase was minimal on using the mixture of antioxidants after cold storage of spermatozoa for 72 hours at 5 °C. The results of frozen thawed stallion se- men revealed that viability indices were superior when using the mixture of antioxidants specially when caffeine was added to the thawing solution. | ||||
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