Topical Photodynamic Therapy Mediated with Hematoporphyrin-HCl using Glycerin as Penetrating Enhancer: In vitro and in vivo studies | ||
| Journal of Laser Science and Applications | ||
| Article 4, Volume 1, Issue 1, July 2024, Pages 5-9 PDF (316 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jlsa.2024.369418 | ||
| Authors | ||
| Abdullah Ibrahim El-Kholy* 1; MAHA Fadel* 2 | ||
| 1Pharmaceutical technology unit, National Institute of Laser Enhanced Sciences (NILES), Cairo University | ||
| 2Dept. Medical Applications of Laser, Pharmaceutical Nanotechnology unit, National Institute of Laser Enhanced Science , Cairo University | ||
| Abstract | ||
| Purpose: In this study, the efficacy of a gel formulation containing Hp-HCl (a photosensitizer) dissolved in glycerin was evaluated for photodynamic treatment (PDT) of tumors. Methods: The release kinetics of water-dissolved Hp-HCl and glycerin-dissolved Hp-HCl from gel formulae were studied in vitro, where the latter showed faster drug release. The growth rates of tumor size in animals, from different treatment groups, were measured. Results: The group treated with the glycerin-dissolved Hp-HCl showed more significant decrease in tumor rate (smaller size) in comparison to the water-dissolved formula, after 35 post-implantation days. Histopathological examination of tumor sections revealed that glycerin-dissolved Hp-HCl effectively distributed the drug throughout the tumor, resulting in almost complete disappearance of tumor cells. Overall, the dissolution of Hp-HCl in glycerin not only increased drug release from the gel formulation but also improved its distribution and therapeutic efficiency. These findings were supported by histopathological results Conclusion: Suggesting that the topical use of Hp-HCl dissolved in glycerin holds promise for improved PDT. | ||
| Keywords | ||
| Photodynamic therapy; Tumor; Topical; Glycerin-Hp-HCl | ||
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