Isolation and characterization of avian pathogenic Escherichia coli from broiler chickens in some governorates in Egypt
Amer, M., Bastamy, M., Ibrahim, H., Salim, M. (2015). Isolation and characterization of avian pathogenic Escherichia coli from broiler chickens in some governorates in Egypt. EKB Journal Management System, 61(1), 1-6. doi: 10.21608/vmjg.2015.369470
M Amer; M Bastamy; H Ibrahim; Mervit M Salim. "Isolation and characterization of avian pathogenic Escherichia coli from broiler chickens in some governorates in Egypt". EKB Journal Management System, 61, 1, 2015, 1-6. doi: 10.21608/vmjg.2015.369470
Amer, M., Bastamy, M., Ibrahim, H., Salim, M. (2015). 'Isolation and characterization of avian pathogenic Escherichia coli from broiler chickens in some governorates in Egypt', EKB Journal Management System, 61(1), pp. 1-6. doi: 10.21608/vmjg.2015.369470
Amer, M., Bastamy, M., Ibrahim, H., Salim, M. Isolation and characterization of avian pathogenic Escherichia coli from broiler chickens in some governorates in Egypt. EKB Journal Management System, 2015; 61(1): 1-6. doi: 10.21608/vmjg.2015.369470

Isolation and characterization of avian pathogenic Escherichia coli from broiler chickens in some governorates in Egypt

Veterinary Medical Journal (Giza)
Volume 61, Issue 1, January 2015, Pages 1-6    PDF (3.55 M)
DOI: 10.21608/vmjg.2015.369470
Authors
M Amer* 1; M Bastamy1; H Ibrahim2; Mervit M Salim3
1Department of Poultry and Rabbits Diseases, Faculty of Veterinary Medicine, Cairo University
2Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
3Master degree student
Abstract
A total of 80 samples (liver, heart, lung, and gall bladder) were collected from diseased broiler chickens from different poultry farms reared in (Zagazig) Sharkia, Ismalia, Sinai, (Dahshor) Giza and Kaluobaia Governorates for bacteriological examination for E. coli infection. The results revealed that 49 out of 80 samples (61%) were positive for E. coli isolation. The result of in vitro Pathogenicity testing by Congo red (CR) binding assay indicates that 14 isolates (28.6%) were CR positive. The most predominant serotypes were O125, O114, and 044 as each represented (2) isolates out of (14) followed by O78, O86, O158, O127, O91, O25, O119, and untypable as each represented (1) isolate out of (14) isolates. Now in this study, we are straining and updating available circulating strains of avian pathogenic Escherichia coli (APEC) micro-organisms to control colibacillosis in broiler chicken either by treatment or vaccination. 
Keywords
Pathogenic; E. coli; Broiler; Isolation; Congo red assay
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