Reveal of Antimicrobial Effect of Lawsonia inermis Extract on Candida albicans Using Molecular and Biochemical Techniques | ||||
| Journal of Basic and Environmental Sciences | ||||
| Volume 1, Issue 2, April 2014, Page 32-45 PDF (374.37 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbes.2014.369571 | ||||
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| Authors | ||||
I. Abulyazid1; G. Singer2; Ragaa M. Ahmed  1
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| 1Chemistry Department, Faculty of Science, Helwan University, Egypt | ||||
| 2Chemistry Department, Faculty of Science, Helwan University, Egypt. | ||||
| Abstract | ||||
| Leaf samples of Lawsonia inermis(Li) was examined to their antimicrobial potential. Broth extracts in different concentrations were prepared and bioassayed in vitro for the growth of Candida albicans. The growth of Candida albicans pathogen was decreased. In addition to the observed alterations which detected electrophoretically in the protein pattern and activity of the catalase enzyme. The protein pattern has one common band of Rf 0.28 and one characteristic band of Rf 0.48 for Candida albicans sample. The obvious quantitative mutation was observed in the fungi with 5, 10, 15% of Lawsonia inermis compared with the control. Some types of proteins in Candida albicans were completely disappeared after inoculated with 5, 10, 15% Li. The catalase pattern showed one common band at R1 with Rf 0.067 and the characteristic bands observed at R5 with Rf 0.42 and at R6 with Rf 0.47 for Candida albicans inoculated with broth. These results confirmed the antifungal activity of henna leaves and supported the traditional use of the plant in therapy of bacterial infections. Disturbances occurred at the biochemical level. The broth extract of the Lawsonia inermis leaves showed obvious antifungal activity against Candida albicans. | ||||
| Keywords | ||||
| Henna; Lawsonia inermis; Medicinal plants Antimicrobial activity Protein pattern; Catalase | ||||
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