Role of β-Hydroxy-γ-Trimethylammonium Butyrate (L-carnitine) and Ubiquinone (CoQ10) in Combating the Deteriorative Effect of Halogenated Alkanes in Liver | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 2, Volume 28, Issue 2, December 2008, Pages 9-22 PDF (212.09 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2008.37012 | ||
| Authors | ||
| Laila Faddah* 1; Atef Abd El-Baky1; Sanaa Ibrahim2; Asmaa Bayoumi1 | ||
| 1Biochemistry Department Faculty of Pharmacy Minia University | ||
| 2Therapeutical Chemistry Department National Research Center El-Dokki, Egyp | ||
| Abstract | ||
| Liver cirrhosis is one of the most dangerous health problems which results from various disorders that damage liver cells over time. The purpose of the current report was carried out to provide information about the liver injury induced by halogenated alkanes (CCl4), and to determine the influence of administration of L-carnitine or/and CoQ10as prophylactic agents against that injury. The study was carried out on 80 adult male albino rats divided into eight groups, 10 animals of each, as follows: four normalgroups (control, treated with L-carnitine, CoQ10, and a combination of L-carnitine and CoQ10) and four cirrhoticgroups treated with CCl4(control, pretreated with L-carnitine, CoQ10, and a combination of L-carnitine and CoQ10). Liver cirrhosis was induced by s.c. injection of a single dose of CCl4(1 ml/kg). L-carnitine (50 mg/kg/day) was given i.p. for four successive days 24 hours before CCl4injection, and CoQ10was given as single i.p. dose (200 mg/kg) 24 hours before CCl4injection. Animals were sacrificed 24 hours after CCl4injection, blood samples were withdrawn and liver tissue samples were homogenized. The studied parameters were: hepatic reduced glutathione (GSH), lipid peroxides, ALT and AST activities, total protein, lactate dehydrogenase (LDH) activity in both serum (with its isozymes) and liver tissues. The injection of CCl4produced a significant decrease in reduced glutathione content and total protein levels in serum and liver cells. However, there was a significant increase in serum ALT and AST activities, lipid peroxides, serum and hepatic total LDH and serum LDH isoenzymes. On the other hand, groups treated with L-carnitine or/and CoQ10(prior to CCl4 injection) showed improvement in most parameters when compared with cirrhotic control group. It has been concluded that L-carnitine and coenzyme Q10have a pronounced prophylactic effect against liver damage induced by halogenated alkanes. | ||
| Keywords | ||
| Coenzyme Q10; halogenated alkanes; L-carnitine; lipid peroxides; LDH and reduced glutathione | ||
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