Characterization and development of a phage cocktail for Escherichia coli causing gastrointestinal diseases | ||||
| Journal of Basic and Environmental Sciences | ||||
| Volume 5, Issue 2, April 2018, Page 115-122 PDF (421.83 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbes.2024.370440 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mohamed A. Nasr-Eldin1; Sabah A. Abo EL-Maaty1; Khaled A. EL-Dougdoug2; Mahmoud M. Hazaa1; Asmaa H. Abdel-mageed1 | ||||
| 1Department of Botany, Faculty of Science, Benha University, Benha, Egypt | ||||
| 2Department of Agric. Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt | ||||
| Abstract | ||||
| Escherichia coli (E. coli) is one of the most important causes of gastrointestinal diseases in developing countries. It considered the third cause of diarrhea in children. In this study, bacteriophages were isolated and detected from wastewater against isolates of E. coli by using plaque and spot test assay. Characterized three phages were differed on plaque morphology, particle morphology, host range and titration. According to phage characterization, the three phages were classified into two from Siphoviridae and one from Podoviridae. Combinations of the bacteriophages had broad host range with strong lytic activity against pathogenic E. coli. Phage cocktail revealed high stability at 45°C, pH 9 and 20% of salt concentration. According to the previous characters of a phage cocktail properties can broaden the spectrum of application of phage therapy. Formulated phages were prepared by mixing phages with royal gelly, dried skimmed milk and a mixture of sucrose and corn flour at ratio 1:1(V: W) at phage cocktail concentration 2.2x1011 plaque forming units (pfu) per mL. These phages formulation remained active for at least 48 days when stored at 4°C in a light protected container with titres of 4.0x1010, 3.6x1010 and 3.4x1010 pfu/mL for royal gelly, dried skimmed milk and a mixture of corn flour and sucrose respectively. | ||||
| Keywords | ||||
| bacteriophage; E. coli; Siphoviridae; Podoviridae; phage cocktail; formulated phage | ||||
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