UPLC-QTOF-MS/MS Based Identification of Bioactive Compounds from Garcinia livingstonei Leaves and Evaluation of their Antioxidant and Antiarthritic Activities | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 68, Issue 2, February 2025, Page 243-261 PDF (801.54 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2024.279689.9518 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Fatma Hassan Nossier  1; Azza Ramy Abdelmonem2; Rehab Ashour3; Marwa Abdel-Aziz4; Ahmed Al_karmalawy5; Ali Elsayed6
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| 1Pharmacognosy department, faculty of pharmacy, Cairo, Egypt | ||||
| 2Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt | ||||
| 3Department of Pharmacognosy,Faculty of Pharmacy, Cairo University | ||||
| 4Naser City, Cairo, Egypt | ||||
| 5Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta 34518, Egypt. | ||||
| 6Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr El-Ainy Street, Cairo 11562, Egypt. | ||||
| Abstract | ||||
| Garcinia livingstonei T. Anderson (family Clusiaceae) is a rich source of different classes of bioactive compounds, mainly phenolics, which possess beneficial pharmacological activities. In the present study the methanolic extract (ME) of the leaves was analyzed using UPLC-QTOF-MS/MS technique in both negative and positive modes. Its total phenolics and flavonoids content were determined using Folin-ciocalteu and Aluminum chloride methods, respectively. Besides, the antioxidant activity of the ME and its different factions was estimated using three different assays (DPPH, FRAP and ABTS), also the antiarthritic activity was evaluated using protein denaturation, membrane stabilization and xanthine oxidase inhibitory % assays. Moreover, two different molecular docking studies were performed to investigate the potential antioxidant and antiarthritic activities of the identified compounds. UPLC-QTOF-MS/MS analysis revealed the presence of biflavonoids, benzophenones, flavonoids, xanthones, phenolic and organic acids. The total phenolics content was found to be 114.72 mg/g extract expressed as gallic acid equivalence, while the total flavonoids content recorded 53.1 mg/g extract expressed as rutin equivalence. Ethyl acetate fraction showed the most potent antioxidant and antiarthritic activities. | ||||
| Keywords | ||||
| Garcinia livingstonei; UPLC-QTOF-MS/MS analysis; antioxidant; antiarthritic; docking | ||||
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