THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 47, Issue 1, January 1999, Pages 89-96 PDF (3.02 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.1999.370634 | ||
| Author | ||
| A DERBALA | ||
| Parasitology and Animal Diseases Dept., National Research Centre, Dokki, Giza, Egypt. | ||
| Abstract | ||
| Fractionation of somatic Voxexara — vitulorwmn antigen was carricd out using Sepharose 4 B gel filtration technqiue. The obtained results indicated that 4 fractions were recovered . F1-F4. All fractions were tested for their antigenicity by agar gel diffusion and ELISA tests. ELISA values of F1 & F2 were relatively similar, while F4 had no any reactivity with agar gel diffusion and ELISA tests. Fraction 2 was usde as identified specific antigen. This anti evaluated for its diagnostic applicability against sera of infected and non infected buffalo-calves by ELISA test. The sensitivity of the test was 100% in diagnosis of confirmed infected calves. Moreover, this test could detect 38.96% of coprologically examined negative calves. It was noticed that the immune response was increased as the intensity of infection had increased. | ||
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