THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES
DERBALA, A. (1999). THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES. EKB Journal Management System, 47(1), 89-96. doi: 10.21608/vmjg.1999.370634
A DERBALA. "THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES". EKB Journal Management System, 47, 1, 1999, 89-96. doi: 10.21608/vmjg.1999.370634
DERBALA, A. (1999). 'THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES', EKB Journal Management System, 47(1), pp. 89-96. doi: 10.21608/vmjg.1999.370634
DERBALA, A. THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES. EKB Journal Management System, 1999; 47(1): 89-96. doi: 10.21608/vmjg.1999.370634

THE USE OF IDENTIFIED SPECIFIC ANTIGEN FOR SERODIAGNOSIS OF TOXOCARA VITULORUM INFECTION IN BUFFALO – CALVES

Veterinary Medical Journal (Giza)
Volume 47, Issue 1, January 1999, Page 89-96  XML PDF (3.02 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.1999.370634
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Author
A DERBALA
Parasitology and Animal Diseases Dept., National Research Centre, Dokki, Giza, Egypt.
Abstract
Fractionation of somatic Voxexara — vitulorwmn antigen was carricd out using Sepharose 4 B gel filtration technqiue. The obtained results indicated that 4 fractions were recovered . F1-F4. All fractions were tested for their antigenicity by agar gel diffusion and ELISA tests. ELISA values of F1 & F2 were relatively similar, while F4 had no any reactivity with agar gel diffusion and ELISA tests. Fraction 2 was usde as identified specific antigen. This anti evaluated for its diagnostic applicability against sera of infected and non infected buffalo-calves by ELISA test. The sensitivity of the test was 100% in diagnosis of confirmed infected calves. Moreover, this test could detect 38.96% of coprologically examined negative calves. It was noticed that the immune response was increased as the intensity of infection had increased.
 
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