Assessment of Interleukin-22 mRNA as a Diagnostic Marker for Polycystic Ovary Syndrome -Associated Metabolic Dysfunction | ||
| Zagazig University Medical Journal | ||
| Article 12, Volume 30, Issue 1.5, August 2024, Pages 2059-2066 PDF (895.92 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zumj.2024.305018.3481 | ||
| Authors | ||
| Nearmeen Rashad* 1; Yaser Samir Saraya2; Eman Abdel Aziz3; Amira Mokhtar Gobran4; Rehab Atef5; Azza Hassan Abd El-Fatah6; mayada mousa7 | ||
| 1Diabetes and Endocrine Unit -Internal Medicin Departement -Zagazig University | ||
| 2Gynecology and Obstetrics, Faculty of Medicine, Zagazig University | ||
| 3clinical pathology department, faculty of medicine, Zagazig university | ||
| 4phsiology department . faculty of medicine zagazig university | ||
| 5Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt Biochemistry Department, College of Medicine, Taif University, Taif, Saudi Arabia | ||
| 6Lecturer of Internal Medicine, Faculty of Medicine, Zagazig University | ||
| 7zagazig university | ||
| Abstract | ||
| Abstract Background: Metabolic abnormalities and immune dysregulations are the most common risk factors for polycystic ovary syndrome (PCOS). Interleukin-22 (IL-22) is implicated in the pathogenesis of the immune-inflammatory system in the context of metabolic dysfunction. In the current research, we aimed to investigate IL-22 mRNA in PCOS and explore its correlations with metabolic syndrome (MetS) and reproductive features of PCOS. Methods: we conducted our research on 60 women as control groups, and 70 patients with PCOS (30 patients without MetS and 40 patients with MetS). We investigated serum IL-22 by ELISA. At the same time, the IL-22 mRNA level was analyzed by RT-qPCR. Results: Among 70 women with PCOS, 40 (57.1%) had metabolic syndrome. women with PCOS had significantly lower serum and mRNA Expression Levels of IL-22 compared to control. Even more importantly, among PCOS patients, women with MetS had significantly lower serum and mRNA Expression levels of IL-22 compared to women without MetS. serum and mRNA Expression levels of IL-22 were negatively associated with MetS components and inflammatory markers as well as reproductive features of PCOS. The AUC of serum and mRNA levels of IL-22 in the prediction of MetS among PCOS women had a sensitivity of 90%, and 77.5%, respectively, and specificity of 66%, 87.3% and respectively. Conclusion: PCOS patients had significantly serum and mRNA Expression levels of IL-22 than controls. the lowered IL-22 serum and mRNA levels could be used as diagnostic markers of PCOS patients more specifically women with MetS. | ||
| Keywords | ||
| Interleukin-22; inflammatory markers; polycystic ovary syndrome; metabolic syndrome; reproductive phenotypes, | ||
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