CLONING AND EXPRESSION OF 3CD GENES OF FMDV | ||
Veterinary Medical Journal (Giza) | ||
Volume 47, Issue 3, July 1999, Pages 289-294 PDF (1.79 M) | ||
Document Type: Original Article | ||
DOI: 10.21608/vmjg.1999.370851 | ||
Authors | ||
M SHAWKY1; M SABER2; A ABDEL RAHMAN1; A EL-SONOUSI1 | ||
1FMD Department, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo. | ||
2Faculty of Veterinary Medicine, Cairo University. | ||
Abstract | ||
RT-PCR amplification of VIA (3D) gene and protease (3C) using its primers. Legation of the PCR product with the cloning vector (using pET system). Expression of the re- combinant plasmid in E. coli expression host. Use of the expressed VIA protein in diagnosis of cattle infected with any of the 7 serotypes of FMD virus with the help of ELISA. | ||
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