Biochemical, Molecular and Genetic Studies on Human Male Fertility | ||||
| Journal of Basic and Environmental Sciences | ||||
| Volume 6, Issue 3, July 2019, Page 209-213 PDF (190.12 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbes.2019.371096 | ||||
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| Authors | ||||
| M.A. Rashed1; S.H. Abd Elaziz2; M.A.Abd Elhafez Salam1; M.H. Awwad2; Nahla Kamel | ||||
| 1Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Egypt | ||||
| 2Faculty of science, Benha University, Benha, Egypt | ||||
| Abstract | ||||
| The present study was carried out at Elnada fertility centre (Almaadi,Cairo,Egypt) and in cooperation with the molecular genetics laborarory Faculty of Agriculture Ain Shams Uninersity, Shoubra El-Kheima, Egypt during the period from 2015 to 2018. determining the nucleotide sequences responsible for human male fertility using specific PCR technique. detection of Y chromosome microdeletions in AZFa, AZFb regionsperformed by evaluation of fresh semen characteristics for sperm DNA fragmentation test and staining method. No correlation observed between sperm concentration, motility, vitality and tail midpiece defects. Negative correlation between sperm DNA fragmentation and acrosome defects and therefore a negative correlation with abnormal forms of sperm. 15 men were screened for submicroscopic Y-chromosome deletions. By using specific PCR technique Y chromosome microdeletion analysis revealed no microdeletion in AZFa, AZFb regions. PCR amplification produced an expected size for AZFa prox-2, SY 127 and AZFa dist-1 loci. So that, the cause of infertility is largely unkown and associated Y chromosome microdeletions cannot be ruled out. | ||||
| Keywords | ||||
| infertility; male infertility; DNA Fragmentation; SRY; microdeletions; AZF region; Y chromosome | ||||
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