Utilizing Start Codon Targeted (SCoT) markers for DNA fingerprinting and diversity analysis of Artemisia herba-alba plant species | ||||
| Journal of Basic and Environmental Sciences | ||||
| Volume 7, Issue 1, January 2020, Page 66-71 PDF (348.75 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbes.2020.371146 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mohamed A. Omar1; Mahmoud Saleh2; Gehad Genidy Mohamed3; Maram El Demerdash4 | ||||
| 1Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt. | ||||
| 2Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, Egypt | ||||
| 3Department of Chemistry, Faculty of Science, Cairo University, 12613, Giza, Egypt. Egypt Nanotechnology Center, Cairo University, El-Sheikh Zayed, 6th October 12588, Giza, Egypt | ||||
| 4Department of Chemistry, Faculty of Science, Cairo University, 12613, Giza, Egypt. | ||||
| Abstract | ||||
| Artemisia herba-alba, is a profoundly significant medicinal plant, known for the abundant spectrum of bioactive metabolites. Its potential for therapeutic and medicinal applications is well established, both in modern and traditional medicine. Research was focused towards the identification of the chemical composition for specimens of A. herba-alba, but not the genetic diversity present within the same species. In this paper, replicate samples of the plant species were collected from three distinct locations in both Mersa-Matrouh and Saint Catherine (Wadi El Raha). Genomic DNA was extracted from aerial plant materials and amplified via SCoT-PCR to determine their genetic relatedness using Dice coefficient to construct their dendrogram. SCoT-PCR associations indicated genetic variance within the species and close association between (S2 & S3) and (M1 & M2). | ||||
| Keywords | ||||
| SCoT PCR; Artemisia herba-alba; Medicinal plants; Secondary metabolites | ||||
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