EVALUATION OF LOCALLY PREPARED EGG AND TISSUE CULTURE ADAPTED FOWL POX VACCINES FROM WP1 STRAIN | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 47, Issue 4, October 1999, Page 499-517 PDF (6.99 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.1999.371420 | ||||
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| Authors | ||||
| M BASTAMY’1; MANAL AFIFY1; SOAD SOLIMAN2; NAMAA MOHAMED2 | ||||
| 1Fac. of Vet. Med. Cairo University, Poultry Department. | ||||
| 2Vet. Scrum and Vaccine Research Institute, Abbasia, Cairo. | ||||
| Abstract | ||||
| Fowl pox virus WPI strain was Passaged to 6 passage on SPE embryonated chicken eggs (ECE) and chicken embryo fibroblasts (CEF) for vaccine preparation. The maximum titre of the inoculated cultures were around the 5th and on the 4th day after ECE and CEF inoculation respectively. The prepared vaccines were safe, sterile and the best temperature for virus preservation was -20C . To compare the efficacy of the locally prepared vaccines with imported commercial vaccines; challenge of vaccinated birds by virulent virus was done which revealed Protection 96% for ECE vaccine and 92% for CFE adapted vaccine. Moreover; seroconversion using ELISA and SNT proved better immunogenicity of the local ECE vaccine over the imported or CEF locally vaccines. | ||||
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