DETECTION OF ANTIGENIC VARIATION AMONG MYCOPLASMA STRAINS ISOLATED FROM TISSUE CULTURES ACCOMPANIED WITH PREPARATION OF SPECIFIC ANTISERA TO P45 AND P49
EL-SHATER, S., EISSA, S., EL-KHATIB, N. (1994). DETECTION OF ANTIGENIC VARIATION AMONG MYCOPLASMA STRAINS ISOLATED FROM TISSUE CULTURES ACCOMPANIED WITH PREPARATION OF SPECIFIC ANTISERA TO P45 AND P49. EKB Journal Management System, 42(2), 113-117. doi: 10.21608/vmjg.1994.371685
S EL-SHATER; S EISSA; NAHLA EL-KHATIB. "DETECTION OF ANTIGENIC VARIATION AMONG MYCOPLASMA STRAINS ISOLATED FROM TISSUE CULTURES ACCOMPANIED WITH PREPARATION OF SPECIFIC ANTISERA TO P45 AND P49". EKB Journal Management System, 42, 2, 1994, 113-117. doi: 10.21608/vmjg.1994.371685
EL-SHATER, S., EISSA, S., EL-KHATIB, N. (1994). 'DETECTION OF ANTIGENIC VARIATION AMONG MYCOPLASMA STRAINS ISOLATED FROM TISSUE CULTURES ACCOMPANIED WITH PREPARATION OF SPECIFIC ANTISERA TO P45 AND P49', EKB Journal Management System, 42(2), pp. 113-117. doi: 10.21608/vmjg.1994.371685
EL-SHATER, S., EISSA, S., EL-KHATIB, N. DETECTION OF ANTIGENIC VARIATION AMONG MYCOPLASMA STRAINS ISOLATED FROM TISSUE CULTURES ACCOMPANIED WITH PREPARATION OF SPECIFIC ANTISERA TO P45 AND P49. EKB Journal Management System, 1994; 42(2): 113-117. doi: 10.21608/vmjg.1994.371685

DETECTION OF ANTIGENIC VARIATION AMONG MYCOPLASMA STRAINS ISOLATED FROM TISSUE CULTURES ACCOMPANIED WITH PREPARATION OF SPECIFIC ANTISERA TO P45 AND P49

Veterinary Medical Journal (Giza)
Volume 42, Issue 2, April 1994, Page 113-117  XML PDF (1.65 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.1994.371685
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Authors
S EL-SHATER* 1; S EISSA2; NAHLA EL-KHATIB2
1Serum and Veterinary Vaccines Institute, Abbassia, Cairo
2Animal Health Research Institute, Dokki, Giza.
Abstract
Comparison of polypeptide patterns of five mycoplasma strains isolated from tissue cultures (M. orale, M. arginini, M. salivarium, M. hyorhinis and A. laidlawii using sodium dodecyl sulphat polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis was carried out. The SDS-PAGE protein profiles of mycoplasmas isolated from tissue cultures showed two common antigens at 45 and 49 kilodaltons (P45 and P49). The immunoreactivity in Western blots of the Strains using M. arginini and M. hyorhinis anti-rabbit sera proved that P45 was found to be more immunogenic than p49. Triton X-114 phase partitioning has revealed the hydrophilic nature of P45 and P49. Antisera raised to electroeluted P45 and P49 reacted specifically with these proteins when assessed on immunoblots of the examined five mycoplasma strains.
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